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@ARTICLE{Schmachtel:304579,
      author       = {T. Schmachtel and H. Bonig and M. A. Rieger$^*$},
      title        = {{FACS}-{B}ased {A}ssessment of {H}uman {H}ematopoietic
                      {S}tem and {P}rogenitor {C}ells.},
      journal      = {International journal of molecular sciences},
      volume       = {26},
      number       = {17},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {DKFZ-2025-01902},
      pages        = {8381},
      year         = {2025},
      abstract     = {The existing heterogeneity of the human hematopoietic stem
                      cell (HSC) compartment imposes significant challenges in
                      understanding their physiology and molecular constitution.
                      The hematopoietic system is hierarchically organized, with
                      HSCs at the apex, responsible for maintaining homeostasis by
                      ensuring a life-long supply of blood cells. HSCs are highly
                      potent but rare, making their pure isolation challenging. To
                      address this, flow-cytometry-based methods are commonly used
                      to isolate HSCs, bridging the gap between surface marker
                      expression and understanding their functional and molecular
                      properties. However, detailed methodology papers providing
                      practical guidance for the prospective isolation of distinct
                      human hematopoietic stem and progenitor cell (HSPC)
                      populations are rare, hindering reproducible applications
                      across different research groups. Here, we present a
                      comprehensive protocol for isolating multipotent long-term
                      repopulating HSCs (LT-HSCs) and define multipotent
                      progenitor populations (MPPs) from human mobilized
                      peripheral blood (mPB) after leukapheresis using
                      fluorescence-activated cell sorting (FACS). By highlighting
                      the workflow, outlining critical considerations and
                      emphasizing recent advancements in the field, we provide an
                      extensive overview of FACS-based human HSC isolation. This
                      facilitates the enrichment of these rare cells for
                      downstream analysis and enables researchers to improve our
                      understanding of the heterogeneity within the HSC
                      compartment.},
      keywords     = {HSPCs (Other) / cell sorting (Other) / flow cytometry
                      (Other) / single cell (Other)},
      cin          = {FM01},
      ddc          = {540},
      cid          = {I:(DE-He78)FM01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40943304},
      pmc          = {pmc:PMC12429214},
      doi          = {10.3390/ijms26178381},
      url          = {https://inrepo02.dkfz.de/record/304579},
}