TY  - JOUR
AU  - Schmidt, Katja
AU  - Butt, Julia
AU  - Matt, Ulrich
AU  - Vogel, Klaus
AU  - Mehl, Calvin
AU  - Ulrich, Rainer G
AU  - Eisenberg, Tobias
TI  - Multiplex serology for Streptobacillus moniliformis and other 'rat bite fever-like' microorganisms for seroprevalence studies in rodents.
JO  - PLOS ONE
VL  - 20
IS  - 10
SN  - 1932-6203
CY  - San Francisco, California, US
PB  - PLOS
M1  - DKFZ-2025-02139
SP  - e0333888
PY  - 2025
N1  - #EA:W440#
AB  - Rat bite fever (RBF) is a zoonotic disease caused primarily by Streptobacillus (S.) moniliformis. Norway or brown rats (Rattus [R.] norvegicus) are the natural host for S. moniliformis and carry the bacterium in the nasopharynx without clinical disease. Transmission to humans often occurs through rat bites or scratches, but also through contact with the excreta of infected rats. Although human infections with S. moniliformis occur worldwide, they are rarely diagnosed. For decades, S. moniliformis was the only known member of the genus Streptobacillus. In recent years, however, four additional species were identified, two of which being zoonotic pathogens capable of causing symptoms identical to RBF in humans. The aim of this study was to develop a serological assay covering all known Streptobacillus species. A bead-based multiplex fluorescence immunoassay for S. moniliformis detection has been used for years in routine diagnostics of laboratory rodents. Here, this assay was adapted to enable the detection of antibodies against all currently known Streptobacillus species and tested with sera from experimentally inoculated mice and rats, and with negative sera from laboratory rodents. Using this assay, we broadly examined the prevalence of Streptobacillus spp. reactive antibodies in wild rodents. Transudates from a total of 107 Norway rats, 81 black or roof rats (Rattus rattus) and 110 house mice (Mus musculus) from different husbandries and wildlife populations within Germany were tested. Antibody prevalences of 41
KW  - Animals
KW  - Streptobacillus: immunology
KW  - Streptobacillus: isolation & purification
KW  - Rats
KW  - Rat-Bite Fever: epidemiology
KW  - Rat-Bite Fever: microbiology
KW  - Rat-Bite Fever: blood
KW  - Rat-Bite Fever: immunology
KW  - Rat-Bite Fever: diagnosis
KW  - Seroepidemiologic Studies
KW  - Mice
KW  - Antibodies, Bacterial: blood
KW  - Humans
KW  - Serologic Tests: methods
KW  - Antibodies, Bacterial (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:41091685
DO  - DOI:10.1371/journal.pone.0333888
UR  - https://inrepo02.dkfz.de/record/305402
ER  -