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| Home > Institute Collections > W500 > Isolation of Murine Myeloid Progenitor Populations by CD34/CD150 Surface Markers. |
| Home > Institute Collections > W500 > Isolation of Murine Myeloid Progenitor Populations by CD34/CD150 Surface Markers. |
| Journal Article | DKFZ-2025-02406 |
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2022
MDPI
Basel
Abstract: Myeloid progenitors are intermediates between Hematopoietic Stem Cells (HSCs) and Myeloid effector progeny. In mouse bone marrow, they are part of the Lineage- cKit+ Sca1- (LK) compartment. To date, most researchers used CD34 and FcγR surface markers for the dissection of this compartment into various populations. Surprisingly, however, this approach does not provide distinct separation by fluorescence-activated cell sorting (FACS). In this study, we suggest using CD150 instead of FcγR. We re-analyzed published single-cell RNA-Seq data and found that CD34/CD150 provides better sub-populations separation, compared to the 'classical' CD34/FcγR-based approach. We confirm our findings by independent FACS analysis. We demonstrate comparable differentiation potential of the newly-obtained LK sub-populations, like previous 'classical' ones. Therefore, we suggest the CD34/CD150 gating strategy, utilizing commonly-used surface markers, as a robust and reproducible separation of the LK compartment into distinct sub-populations.
Keyword(s): Animals (MeSH) ; Antigens, CD34: metabolism (MeSH) ; Cell Adhesion Molecules: metabolism (MeSH) ; Flow Cytometry (MeSH) ; Hematopoietic Stem Cells: metabolism (MeSH) ; Mice (MeSH) ; Myeloid Progenitor Cells (MeSH) ; Receptors, IgG: metabolism (MeSH) ; Signaling Lymphocytic Activation Molecule Family Member 1: metabolism (MeSH) ; cell sorting ; hematopoiesis ; murine bone marrow ; myeloid progenitors ; Antigens, CD34 ; Cell Adhesion Molecules ; Receptors, IgG ; SLAMF1 protein, human ; Signaling Lymphocytic Activation Molecule Family Member 1
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