%0 Journal Article
%A Olender, Leonid
%A Thapa, Roshina
%A Gazit, Roi
%T Isolation of Murine Myeloid Progenitor Populations by CD34/CD150 Surface Markers.
%J Cells
%V 11
%N 3
%@ 2073-4409
%C Basel
%I MDPI
%M DKFZ-2025-02406
%P 350
%D 2022
%Z #DKFZ-MOST-Ca179#
%X Myeloid progenitors are intermediates between Hematopoietic Stem Cells (HSCs) and Myeloid effector progeny. In mouse bone marrow, they are part of the Lineage- cKit+ Sca1- (LK) compartment. To date, most researchers used CD34 and FcγR surface markers for the dissection of this compartment into various populations. Surprisingly, however, this approach does not provide distinct separation by fluorescence-activated cell sorting (FACS). In this study, we suggest using CD150 instead of FcγR. We re-analyzed published single-cell RNA-Seq data and found that CD34/CD150 provides better sub-populations separation, compared to the 'classical' CD34/FcγR-based approach. We confirm our findings by independent FACS analysis. We demonstrate comparable differentiation potential of the newly-obtained LK sub-populations, like previous 'classical' ones. Therefore, we suggest the CD34/CD150 gating strategy, utilizing commonly-used surface markers, as a robust and reproducible separation of the LK compartment into distinct sub-populations.
%K Animals
%K Antigens, CD34: metabolism
%K Cell Adhesion Molecules: metabolism
%K Flow Cytometry
%K Hematopoietic Stem Cells: metabolism
%K Mice
%K Myeloid Progenitor Cells
%K Receptors, IgG: metabolism
%K Signaling Lymphocytic Activation Molecule Family Member 1: metabolism
%K cell sorting (Other)
%K hematopoiesis (Other)
%K murine bone marrow (Other)
%K myeloid progenitors (Other)
%K Antigens, CD34 (NLM Chemicals)
%K Cell Adhesion Molecules (NLM Chemicals)
%K Receptors, IgG (NLM Chemicals)
%K SLAMF1 protein, human (NLM Chemicals)
%K Signaling Lymphocytic Activation Molecule Family Member 1 (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:35159159
%2 pmc:PMC8834359
%R 10.3390/cells11030350
%U https://inrepo02.dkfz.de/record/305789