%0 Journal Article
%A Moerkens, Renée
%A Mooiweer, Joram
%A Smits, Eline
%A Berg, Marijn
%A Ramírez-Sánchez, Aarón D
%A Modderman, Rutger
%A Puschhof, Jens
%A Pleguezuelos-Manzano, Cayetano
%A Barrett, Robert J
%A Wijmenga, Cisca
%A Jonkers, Iris H
%A Withoff, Sebo
%T Gene expression profiling reveals enhanced nutrient and drug metabolism and maturation of hiPSC-derived intestine-on-chip relative to organoids and Transwells.
%J Stem cell reports
%V 20
%N 12
%@ 2213-6711
%C Maryland Heights, MO
%I Cell Press
%M DKFZ-2025-02440
%P 102715
%D 2025
%Z 2025 Dec 9;20(12):102715
%X The human intestinal epithelial barrier is shaped by biological and biomechanical cues, including growth factor gradients and fluid flow. While these factors are known to affect adult stem cell (ASC)-derived intestinal epithelial cells in vitro, their impact on human induced pluripotent stem cell (hiPSC)-derived cells is largely unexplored. Here, we compare the cellular composition and gene expression profiles of hiPSC-derived intestinal epithelial cells exposed to various medium compositions and cultured as organoids, in Transwell and microfluidic intestine-on-chip systems. Modulating key signaling pathways (WNT, NOTCH, bone morphogenetic protein [BMP], and mitogen-activated protein kinase [MAPK]) influenced the presence of dividing, absorptive, and secretory epithelial lineages. Upon differentiation, intestinal epithelial cells expressed genes encoding digestive enzymes, nutrient transporters, and drug-metabolizing enzymes. Notably, these pathways were most enhanced in the intestine-on-chip system, along with an expression profile that suggests a more mature state. These findings highlight the potential of hiPSC-derived intestinal cells to model important intestinal functions and guide the selection of optimal culture conditions for specific applications.
%K Transwell (Other)
%K differentiation medium (Other)
%K gut-on-chip (Other)
%K human (Other)
%K induced pluripotent stem cell (Other)
%K intestinal epithelial barrier (Other)
%K intestine-on-chip (Other)
%K organ-on-chip (Other)
%K organoid (Other)
%K small intestine (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41237783
%R 10.1016/j.stemcr.2025.102715
%U https://inrepo02.dkfz.de/record/306211