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@ARTICLE{Moerkens:306211,
      author       = {R. Moerkens and J. Mooiweer and E. Smits and M. Berg and A.
                      D. Ramírez-Sánchez and R. Modderman and J. Puschhof$^*$
                      and C. Pleguezuelos-Manzano and R. J. Barrett and C.
                      Wijmenga and I. H. Jonkers and S. Withoff},
      title        = {{G}ene expression profiling reveals enhanced nutrient and
                      drug metabolism and maturation of hi{PSC}-derived
                      intestine-on-chip relative to organoids and {T}ranswells.},
      journal      = {Stem cell reports},
      volume       = {20},
      number       = {12},
      issn         = {2213-6711},
      address      = {Maryland Heights, MO},
      publisher    = {Cell Press},
      reportid     = {DKFZ-2025-02440},
      pages        = {102715},
      year         = {2025},
      note         = {2025 Dec 9;20(12):102715},
      abstract     = {The human intestinal epithelial barrier is shaped by
                      biological and biomechanical cues, including growth factor
                      gradients and fluid flow. While these factors are known to
                      affect adult stem cell (ASC)-derived intestinal epithelial
                      cells in vitro, their impact on human induced pluripotent
                      stem cell (hiPSC)-derived cells is largely unexplored. Here,
                      we compare the cellular composition and gene expression
                      profiles of hiPSC-derived intestinal epithelial cells
                      exposed to various medium compositions and cultured as
                      organoids, in Transwell and microfluidic intestine-on-chip
                      systems. Modulating key signaling pathways (WNT, NOTCH, bone
                      morphogenetic protein [BMP], and mitogen-activated protein
                      kinase [MAPK]) influenced the presence of dividing,
                      absorptive, and secretory epithelial lineages. Upon
                      differentiation, intestinal epithelial cells expressed genes
                      encoding digestive enzymes, nutrient transporters, and
                      drug-metabolizing enzymes. Notably, these pathways were most
                      enhanced in the intestine-on-chip system, along with an
                      expression profile that suggests a more mature state. These
                      findings highlight the potential of hiPSC-derived intestinal
                      cells to model important intestinal functions and guide the
                      selection of optimal culture conditions for specific
                      applications.},
      keywords     = {Transwell (Other) / differentiation medium (Other) /
                      gut-on-chip (Other) / human (Other) / induced pluripotent
                      stem cell (Other) / intestinal epithelial barrier (Other) /
                      intestine-on-chip (Other) / organ-on-chip (Other) / organoid
                      (Other) / small intestine (Other)},
      cin          = {D300},
      ddc          = {610},
      cid          = {I:(DE-He78)D300-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41237783},
      doi          = {10.1016/j.stemcr.2025.102715},
      url          = {https://inrepo02.dkfz.de/record/306211},
}