%0 Journal Article
%A Amon, Lukas
%A Vurnek, Damir
%A Seichter, Anna
%A Tchitashvili, Giorgi
%A Kaszubowski, Tomasz
%A Mroz, Markus
%A Debeuf, Nincy
%A Vogler, Tina
%A Küpper, Nicole
%A Rengarajan, Kaushikk Ravi
%A Lächele, Lukas
%A Tochoedo, Nounagnon R
%A Baranska, Anna
%A Autenrieth, Stella E
%A Nimmerjahn, Falk
%A Hildner, Kai
%A Pfeffer, Klaus
%A Schraml, Barbara U
%A Heger, Lukas
%A Lambrecht, Bart N
%A Lehmann, Christian H K
%A Dudziak, Diana
%T GM-CSF and specific type 2 cytokines induce CD103+ and CD301b+ cell states in cDC1s and cDC2s.
%J Cell reports
%V 44
%N 12
%@ 2211-1247
%C Maryland Heights, MO
%I Cell Press
%M DKFZ-2025-02643
%P 116589
%D 2025
%X The heterogeneity of conventional dendritic cells type 1 (cDC1s) and type 2 (cDC2s) is well established, yet the identity and origin of CD301b+ cDC2s remain debated. Here, we show that CD301b+ cDC2s and CD103+ cDC1s develop from pre-committed progenitors in response to granulocyte/macrophage colony-stimulating factor (GM-CSF). While CD103+ cDC1s acquire their phenotype and functional properties through GM-CSF-driven differentiation from pre-cDC1s, CD301b+ cDC2s emerge as cytokine-induced states from DC2- and DC3-committed progenitors. CD103+ cDC1s and CD301b+ cDC2s exhibit enhanced T cell priming capacities and distinct cytokine expression profiles upon GM-CSF exposure. In vivo, DC-intrinsic GM-CSF sensing is dispensable for acquiring CD103 and CD301b expression with the notable exception of lung DCs, while specific type 2 cytokines induce CD103 and CD301b ex vivo. These findings identify GM-CSF and specific type 2 cytokines as central regulators of cDC1 and cDC2 effector differentiation and establish CD301b as a marker of a cytokine-driven cDC2 state.
%K CD103(+) cDC1 (Other)
%K CD301b (Other)
%K CD301b(+) cDC2 (Other)
%K CP: immunology (Other)
%K DC2 and DC3 progenitor (Other)
%K GM-CSF (Other)
%K cell state (Other)
%K conventional dendritic cells (Other)
%K heterogeneity (Other)
%K ontogeny (Other)
%K type 2 cytokines (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41307996
%R 10.1016/j.celrep.2025.116589
%U https://inrepo02.dkfz.de/record/306606