TY  - JOUR
AU  - Amon, Lukas
AU  - Vurnek, Damir
AU  - Seichter, Anna
AU  - Tchitashvili, Giorgi
AU  - Kaszubowski, Tomasz
AU  - Mroz, Markus
AU  - Debeuf, Nincy
AU  - Vogler, Tina
AU  - Küpper, Nicole
AU  - Rengarajan, Kaushikk Ravi
AU  - Lächele, Lukas
AU  - Tochoedo, Nounagnon R
AU  - Baranska, Anna
AU  - Autenrieth, Stella E
AU  - Nimmerjahn, Falk
AU  - Hildner, Kai
AU  - Pfeffer, Klaus
AU  - Schraml, Barbara U
AU  - Heger, Lukas
AU  - Lambrecht, Bart N
AU  - Lehmann, Christian H K
AU  - Dudziak, Diana
TI  - GM-CSF and specific type 2 cytokines induce CD103+ and CD301b+ cell states in cDC1s and cDC2s.
JO  - Cell reports
VL  - 44
IS  - 12
SN  - 2211-1247
CY  - Maryland Heights, MO
PB  - Cell Press
M1  - DKFZ-2025-02643
SP  - 116589
PY  - 2025
AB  - The heterogeneity of conventional dendritic cells type 1 (cDC1s) and type 2 (cDC2s) is well established, yet the identity and origin of CD301b+ cDC2s remain debated. Here, we show that CD301b+ cDC2s and CD103+ cDC1s develop from pre-committed progenitors in response to granulocyte/macrophage colony-stimulating factor (GM-CSF). While CD103+ cDC1s acquire their phenotype and functional properties through GM-CSF-driven differentiation from pre-cDC1s, CD301b+ cDC2s emerge as cytokine-induced states from DC2- and DC3-committed progenitors. CD103+ cDC1s and CD301b+ cDC2s exhibit enhanced T cell priming capacities and distinct cytokine expression profiles upon GM-CSF exposure. In vivo, DC-intrinsic GM-CSF sensing is dispensable for acquiring CD103 and CD301b expression with the notable exception of lung DCs, while specific type 2 cytokines induce CD103 and CD301b ex vivo. These findings identify GM-CSF and specific type 2 cytokines as central regulators of cDC1 and cDC2 effector differentiation and establish CD301b as a marker of a cytokine-driven cDC2 state.
KW  - CD103(+) cDC1 (Other)
KW  - CD301b (Other)
KW  - CD301b(+) cDC2 (Other)
KW  - CP: immunology (Other)
KW  - DC2 and DC3 progenitor (Other)
KW  - GM-CSF (Other)
KW  - cell state (Other)
KW  - conventional dendritic cells (Other)
KW  - heterogeneity (Other)
KW  - ontogeny (Other)
KW  - type 2 cytokines (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:41307996
DO  - DOI:10.1016/j.celrep.2025.116589
UR  - https://inrepo02.dkfz.de/record/306606
ER  -