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@ARTICLE{Amon:306606,
author = {L. Amon and D. Vurnek and A. Seichter and G. Tchitashvili
and T. Kaszubowski and M. Mroz and N. Debeuf and T. Vogler
and N. Küpper and K. R. Rengarajan and L. Lächele and N.
R. Tochoedo and A. Baranska and S. E. Autenrieth$^*$ and F.
Nimmerjahn and K. Hildner and K. Pfeffer and B. U. Schraml
and L. Heger and B. N. Lambrecht and C. H. K. Lehmann and D.
Dudziak},
title = {{GM}-{CSF} and specific type 2 cytokines induce {CD}103+
and {CD}301b+ cell states in c{DC}1s and c{DC}2s.},
journal = {Cell reports},
volume = {44},
number = {12},
issn = {2211-1247},
address = {Maryland Heights, MO},
publisher = {Cell Press},
reportid = {DKFZ-2025-02643},
pages = {116589},
year = {2025},
abstract = {The heterogeneity of conventional dendritic cells type 1
(cDC1s) and type 2 (cDC2s) is well established, yet the
identity and origin of CD301b+ cDC2s remain debated. Here,
we show that CD301b+ cDC2s and CD103+ cDC1s develop from
pre-committed progenitors in response to
granulocyte/macrophage colony-stimulating factor (GM-CSF).
While CD103+ cDC1s acquire their phenotype and functional
properties through GM-CSF-driven differentiation from
pre-cDC1s, CD301b+ cDC2s emerge as cytokine-induced states
from DC2- and DC3-committed progenitors. CD103+ cDC1s and
CD301b+ cDC2s exhibit enhanced T cell priming capacities and
distinct cytokine expression profiles upon GM-CSF exposure.
In vivo, DC-intrinsic GM-CSF sensing is dispensable for
acquiring CD103 and CD301b expression with the notable
exception of lung DCs, while specific type 2 cytokines
induce CD103 and CD301b ex vivo. These findings identify
GM-CSF and specific type 2 cytokines as central regulators
of cDC1 and cDC2 effector differentiation and establish
CD301b as a marker of a cytokine-driven cDC2 state.},
keywords = {CD103(+) cDC1 (Other) / CD301b (Other) / CD301b(+) cDC2
(Other) / CP: immunology (Other) / DC2 and DC3 progenitor
(Other) / GM-CSF (Other) / cell state (Other) / conventional
dendritic cells (Other) / heterogeneity (Other) / ontogeny
(Other) / type 2 cytokines (Other)},
cin = {D431},
ddc = {610},
cid = {I:(DE-He78)D431-20160331},
pnm = {314 - Immunologie und Krebs (POF4-314)},
pid = {G:(DE-HGF)POF4-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:41307996},
doi = {10.1016/j.celrep.2025.116589},
url = {https://inrepo02.dkfz.de/record/306606},
}