TY  - JOUR
AU  - Seifert, Sophie
AU  - Yanqui-Rivera, Francisco
AU  - Kühn, Tim
AU  - Høyland, Lena Elise
AU  - Bortecen, Toman
AU  - Agranovich, Bella
AU  - Eckhardt, Lara
AU  - Herdt, Martin
AU  - Henneberg, Alessa
AU  - Panitz, Verena
AU  - Hayat, Faisal
AU  - Migaud, Marie
AU  - Poschet, Gernot
AU  - Abramovich, Ifat
AU  - Gottlieb, Eyal
AU  - Krijgsveld, Jeroen
AU  - Ziegler, Mathias
AU  - Prentzell, Mirja Tamara
AU  - Opitz, Christiane
TI  - A step-by-step guide to performing cancer metabolism research using custom-made media.
JO  - Life science alliance
VL  - 9
IS  - 2
SN  - 2575-1077
CY  - Heidelberg
PB  - EMBO Press
M1  - DKFZ-2025-02912
SP  - e202503529 -
PY  - 2026
N1  - #EA:B350#LA:B350#
AB  - The preparation of custom-made media offers precise control over nutrient composition, enabling detailed studies of cellular metabolism. We demonstrate how self-made media formulations enable diverse assay designs and readouts to assess cancer metabolism. Self-made media can be used in Seahorse assays to measure mitochondrial respiration under defined conditions. In nutrient deprivation experiments, amino acid or vitamin removal can uncover how cancer cells adapt to metabolic stress. Using labeled amino acids enables analysis of nascent protein synthesis and translational regulation, while stable-isotope tracing reveals metabolic fluxes through key pathways. This guide presents a suite of metabolic assays using custom-made media, covering experimental design, the selection of controls, sample preparation, data acquisition, and interpretation. The accompanying online media calculator 'Media Minds' streamlines the creation of custom media formulations, ensuring accuracy and reproducibility.
KW  - Humans
KW  - Neoplasms: metabolism
KW  - Culture Media: chemistry
KW  - Mitochondria: metabolism
KW  - Cell Line, Tumor
KW  - Amino Acids: metabolism
KW  - Metabolomics: methods
KW  - Culture Media (NLM Chemicals)
KW  - Amino Acids (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:41371957
C2  - pmc:PMC12696398
DO  - DOI:10.26508/lsa.202503529
UR  - https://inrepo02.dkfz.de/record/306940
ER  -