% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Seifert:306940,
      author       = {S. Seifert$^*$ and F. Yanqui-Rivera$^*$ and T. Kühn$^*$
                      and L. E. Høyland and T. Bortecen$^*$ and B. Agranovich and
                      L. Eckhardt$^*$ and M. Herdt$^*$ and A. Henneberg$^*$ and V.
                      Panitz$^*$ and F. Hayat and M. Migaud and G. Poschet and I.
                      Abramovich and E. Gottlieb and J. Krijgsveld$^*$ and M.
                      Ziegler and M. T. Prentzell$^*$ and C. Opitz$^*$},
      title        = {{A} step-by-step guide to performing cancer metabolism
                      research using custom-made media.},
      journal      = {Life science alliance},
      volume       = {9},
      number       = {2},
      issn         = {2575-1077},
      address      = {Heidelberg},
      publisher    = {EMBO Press},
      reportid     = {DKFZ-2025-02912},
      pages        = {e202503529 -},
      year         = {2026},
      note         = {#EA:B350#LA:B350#},
      abstract     = {The preparation of custom-made media offers precise control
                      over nutrient composition, enabling detailed studies of
                      cellular metabolism. We demonstrate how self-made media
                      formulations enable diverse assay designs and readouts to
                      assess cancer metabolism. Self-made media can be used in
                      Seahorse assays to measure mitochondrial respiration under
                      defined conditions. In nutrient deprivation experiments,
                      amino acid or vitamin removal can uncover how cancer cells
                      adapt to metabolic stress. Using labeled amino acids enables
                      analysis of nascent protein synthesis and translational
                      regulation, while stable-isotope tracing reveals metabolic
                      fluxes through key pathways. This guide presents a suite of
                      metabolic assays using custom-made media, covering
                      experimental design, the selection of controls, sample
                      preparation, data acquisition, and interpretation. The
                      accompanying online media calculator 'Media Minds'
                      streamlines the creation of custom media formulations,
                      ensuring accuracy and reproducibility.},
      subtyp        = {Review Article},
      keywords     = {Humans / Neoplasms: metabolism / Culture Media: chemistry /
                      Mitochondria: metabolism / Cell Line, Tumor / Amino Acids:
                      metabolism / Metabolomics: methods / Culture Media (NLM
                      Chemicals) / Amino Acids (NLM Chemicals)},
      cin          = {B350 / HD01 / B230},
      ddc          = {570},
      cid          = {I:(DE-He78)B350-20160331 / I:(DE-He78)HD01-20160331 /
                      I:(DE-He78)B230-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311) / SFB 1389
                      C04 - Metabolische Signalübertragung bei glioblastomen: ein
                      räumlich aufgelöster Multi-Omics-Ansatz (C04) (424568869)},
      pid          = {G:(DE-HGF)POF4-311 / G:(GEPRIS)424568869},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41371957},
      pmc          = {pmc:PMC12696398},
      doi          = {10.26508/lsa.202503529},
      url          = {https://inrepo02.dkfz.de/record/306940},
}