%0 Journal Article
%A Bhave, Rigveda
%A Kath, Carla-Johanna
%A Rüchel, Nadine
%A Vasileiou, Eleni
%A Jepsen, Vera H
%A Raba, Katharina
%A Pandyra, Aleksandra A
%A Fischer, Ute
%A Kogler, Gesine
%T Serum-free differentiation platform for the generation of B lymphocytes and natural killer cells from human CD34+ cord blood progenitors.
%J Scientific reports
%V 15
%N 1
%@ 2045-2322
%C [London]
%I Springer Nature
%M DKFZ-2025-02999
%P 44132
%D 2025
%X Pre-clinical research on B and NK cell development relies on murine stromal cell-based systems with reduced physiological relevance and clinical applicability. A serum-free, fully humanized co-culture system utilizing human bone marrow-derived mesenchymal stromal cells (BM-MSCs) was developed to differentiate CB-CD34+ cells towards B and NK cell lineages. Differentiation dynamics were monitored via flow cytometry, with immunophenotypic analysis tracking progression from progenitors to mature cells. The system generated CD19+ IgM+ immature B cells and CD56+ CD16+ NK cells, recapitulating fetal stages of human lymphopoiesis. Serum-free media conditions ensured reproducibility and high overall yield of CD19+ B (35 ± 5.32
%K B lymphocytes (Other)
%K Cell differentiation (Other)
%K Cord blood (Other)
%K Culture media (Other)
%K Killer cells (Other)
%K Mesenchymal stem cells (Other)
%K Natural (Other)
%K Serum-free (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41413562
%R 10.1038/s41598-025-30732-9
%U https://inrepo02.dkfz.de/record/307313