TY  - JOUR
AU  - Bhave, Rigveda
AU  - Kath, Carla-Johanna
AU  - Rüchel, Nadine
AU  - Vasileiou, Eleni
AU  - Jepsen, Vera H
AU  - Raba, Katharina
AU  - Pandyra, Aleksandra A
AU  - Fischer, Ute
AU  - Kogler, Gesine
TI  - Serum-free differentiation platform for the generation of B lymphocytes and natural killer cells from human CD34+ cord blood progenitors.
JO  - Scientific reports
VL  - 15
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Springer Nature
M1  - DKFZ-2025-02999
SP  - 44132
PY  - 2025
AB  - Pre-clinical research on B and NK cell development relies on murine stromal cell-based systems with reduced physiological relevance and clinical applicability. A serum-free, fully humanized co-culture system utilizing human bone marrow-derived mesenchymal stromal cells (BM-MSCs) was developed to differentiate CB-CD34+ cells towards B and NK cell lineages. Differentiation dynamics were monitored via flow cytometry, with immunophenotypic analysis tracking progression from progenitors to mature cells. The system generated CD19+ IgM+ immature B cells and CD56+ CD16+ NK cells, recapitulating fetal stages of human lymphopoiesis. Serum-free media conditions ensured reproducibility and high overall yield of CD19+ B (35 ± 5.32
KW  - B lymphocytes (Other)
KW  - Cell differentiation (Other)
KW  - Cord blood (Other)
KW  - Culture media (Other)
KW  - Killer cells (Other)
KW  - Mesenchymal stem cells (Other)
KW  - Natural (Other)
KW  - Serum-free (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:41413562
DO  - DOI:10.1038/s41598-025-30732-9
UR  - https://inrepo02.dkfz.de/record/307313
ER  -