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@ARTICLE{Bhave:307313,
      author       = {R. Bhave$^*$ and C.-J. Kath$^*$ and N. Rüchel$^*$ and E.
                      Vasileiou and V. H. Jepsen$^*$ and K. Raba and A. A. Pandyra
                      and U. Fischer$^*$ and G. Kogler},
      title        = {{S}erum-free differentiation platform for the generation of
                      {B} lymphocytes and natural killer cells from human {CD}34+
                      cord blood progenitors.},
      journal      = {Scientific reports},
      volume       = {15},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Springer Nature},
      reportid     = {DKFZ-2025-02999},
      pages        = {44132},
      year         = {2025},
      abstract     = {Pre-clinical research on B and NK cell development relies
                      on murine stromal cell-based systems with reduced
                      physiological relevance and clinical applicability. A
                      serum-free, fully humanized co-culture system utilizing
                      human bone marrow-derived mesenchymal stromal cells
                      (BM-MSCs) was developed to differentiate CB-CD34+ cells
                      towards B and NK cell lineages. Differentiation dynamics
                      were monitored via flow cytometry, with immunophenotypic
                      analysis tracking progression from progenitors to mature
                      cells. The system generated CD19+ IgM+ immature B cells and
                      CD56+ CD16+ NK cells, recapitulating fetal stages of human
                      lymphopoiesis. Serum-free media conditions ensured
                      reproducibility and high overall yield of CD19+ B (35 ±
                      $5.32\%)$ and CD56+ NK (28.46 ± $7.01\%)$ cell progenitors.
                      Flow cytometry identified distinct population peaks,
                      confirming temporal control over differentiation. This
                      clinically relevant platform addresses the limitations of
                      traditional models by providing a more physiologically
                      accurate human microenvironment. The serum-free system
                      supports applications in disease modeling, genotoxic
                      compound screening, and mutational studies of hematopoiesis.
                      By enabling scalable production of B and NK cells it aims to
                      accelerate translational research for immunodeficiencies,
                      cancer immunotherapy, and hematopoietic disorders.},
      keywords     = {B lymphocytes (Other) / Cell differentiation (Other) / Cord
                      blood (Other) / Culture media (Other) / Killer cells (Other)
                      / Mesenchymal stem cells (Other) / Natural (Other) /
                      Serum-free (Other)},
      cin          = {ED01},
      ddc          = {600},
      cid          = {I:(DE-He78)ED01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41413562},
      doi          = {10.1038/s41598-025-30732-9},
      url          = {https://inrepo02.dkfz.de/record/307313},
}