%0 Journal Article
%A Stein, Catarina M
%A Hablesreiter, Raphael
%A Christen, Friederike
%A Löwe, Pelle
%A Fustero-Torre, Coral
%A Kopp, Klara
%A Locher, Benjamin N
%A Nitsch, Lena
%A Altwasser, Robert
%A Kerschbaum, Johanna Franziska
%A Bullinger, Lars
%A Ludwig, Leif S
%A Strzelecka, Paulina M
%A Damm, Frederik
%T Dynamics of clonal hematopoiesis and cellular responses to stress-induced toxicity in autologous stem cell transplantation.
%J Leukemia
%V nn
%@ 0887-6924
%C London
%I Springer Nature
%M DKFZ-2025-03015
%P nn
%D 2025
%Z epub
%X Autologous stem cell transplantation (ASCT) involves harvesting hematopoietic stem and progenitor cells (HSPCs) prior to chemotherapy and subsequent repopulation of the bone marrow. This process imposes a bottleneck, providing a framework to dissect the unresolved short- and long-term clonal dynamics during hematopoietic reconstitution. By integrating bulk error-corrected targeted sequencing of clonal hematopoiesis (CH)-associated genes with mitochondrial single-cell Assay for Transposase-Accessible Chromatin sequencing (mtscATAC-seq), we characterized mutational trajectories in frequently altered hematological genes and traced clonal evolution through somatic mitochondrial DNA variants, revealing post-transplant cellular heterogeneity and clonal architecture. Among 60 patients (multiple myeloma, n = 51; non-Hodgkin lymphoma, n = 6; Hodgkin lymphoma, n = 3), CH-associated mutations were identified in 53
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41419602
%R 10.1038/s41375-025-02823-z
%U https://inrepo02.dkfz.de/record/307371