TY  - JOUR
AU  - Stein, Catarina M
AU  - Hablesreiter, Raphael
AU  - Christen, Friederike
AU  - Löwe, Pelle
AU  - Fustero-Torre, Coral
AU  - Kopp, Klara
AU  - Locher, Benjamin N
AU  - Nitsch, Lena
AU  - Altwasser, Robert
AU  - Kerschbaum, Johanna Franziska
AU  - Bullinger, Lars
AU  - Ludwig, Leif S
AU  - Strzelecka, Paulina M
AU  - Damm, Frederik
TI  - Dynamics of clonal hematopoiesis and cellular responses to stress-induced toxicity in autologous stem cell transplantation.
JO  - Leukemia
VL  - nn
SN  - 0887-6924
CY  - London
PB  - Springer Nature
M1  - DKFZ-2025-03015
SP  - nn
PY  - 2025
N1  - epub
AB  - Autologous stem cell transplantation (ASCT) involves harvesting hematopoietic stem and progenitor cells (HSPCs) prior to chemotherapy and subsequent repopulation of the bone marrow. This process imposes a bottleneck, providing a framework to dissect the unresolved short- and long-term clonal dynamics during hematopoietic reconstitution. By integrating bulk error-corrected targeted sequencing of clonal hematopoiesis (CH)-associated genes with mitochondrial single-cell Assay for Transposase-Accessible Chromatin sequencing (mtscATAC-seq), we characterized mutational trajectories in frequently altered hematological genes and traced clonal evolution through somatic mitochondrial DNA variants, revealing post-transplant cellular heterogeneity and clonal architecture. Among 60 patients (multiple myeloma, n = 51; non-Hodgkin lymphoma, n = 6; Hodgkin lymphoma, n = 3), CH-associated mutations were identified in 53
LB  - PUB:(DE-HGF)16
C6  - pmid:41419602
DO  - DOI:10.1038/s41375-025-02823-z
UR  - https://inrepo02.dkfz.de/record/307371
ER  -