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@ARTICLE{ElHarouni:307373,
author = {D. ElHarouni$^*$ and R. Hernansaiz-Ballesteros and H.
Peterziel$^*$ and G. Balasubramanian$^*$ and C. Previti$^*$
and K. Schramm$^*$ and M. Blattner-Johnson$^*$ and R.
Kabbe$^*$ and B. Jones$^*$ and S. Oppermann$^*$ and D.
Jones$^*$ and S. Pfister$^*$ and O. Witt$^*$ and J.
Saez-Rodriguez and I. Oehme$^*$ and N. Jäger$^*$ and M.
Schlesner},
title = {{I}ntegrative {M}ultiomics and {D}rug {S}ensitivity
{P}rofiling {R}eveal {P}otential {B}iomarkers and
{T}herapeutic {S}trategies in {P}ediatric {S}olid {T}umors.},
journal = {Cancer research},
volume = {nn},
issn = {0099-7013},
address = {Philadelphia, Pa.},
publisher = {AACR},
reportid = {DKFZ-2025-03017},
pages = {nn},
year = {2025},
note = {#EA:B062#LA:B062# / epub},
abstract = {Cure rates for childhood malignancies using established
therapy protocols have increased to an average of $80\%$ but
have reached a plateau. Moreover, survival rates are
particularly low for some pediatric tumors-such as high-risk
group 3 medulloblastomas, osteosarcomas, Ewing sarcomas,
high-risk neuroblastomas, and high-grade gliomas-and dismal
for patients with relapsed malignancies. A functional drug
response profiling platform for pediatric solid and brain
tumors has been established within the INFORM program to
identify patient-specific vulnerabilities and biomarkers and
to unravel molecular mechanisms associated with drug
response profiles for clinical translation. In this study,
we performed a multiomics analysis using drug sensitivity
profiles, as well as genomic and transcriptomic data, of 81
pediatric solid tumor samples. The integrative analysis
suggested two multiomics signatures associated with drug
sensitivity. One signature distinguished neuroblastoma
samples with sensitivity to navitoclax, a BCL2 family
inhibitor. A second signature was specific to a subset of
Wilms tumors harboring the SIX1 (Q177R) hotspot mutation
that displayed high expression of MGAM, PTPN14, STAT4, and
KDM2B and high sensitivity to MEK inhibitors. A
patient-specific causal interaction network analysis
suggested possible molecular interactions between MEK
inhibitors and the SIX1 mutation in Wilms tumor samples. In
conclusion, the integration of drug sensitivity profiling
and multiomics data revealed potential biomarkers that may
be associated with drug sensitivity in pediatric solid
tumors. Patient-specific causal interaction network analysis
further elucidated the interaction between inhibitors and
signature biomarkers, providing insights that may inform
clinical translation.The combination of multiomics analysis
and drug sensitivity profiling identified two signatures
related to drug sensitivity in pediatric solid tumors,
contributing to the advancement of functional precision
medicine and personalized treatment strategies. This article
is part of a special series: Driving Cancer Discoveries with
Computational Research, Data Science, and Machine
Learning/AI .},
cin = {B062 / HD01 / B310 / W610 / B360},
ddc = {610},
cid = {I:(DE-He78)B062-20160331 / I:(DE-He78)HD01-20160331 /
I:(DE-He78)B310-20160331 / I:(DE-He78)W610-20160331 /
I:(DE-He78)B360-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:41417259},
doi = {10.1158/0008-5472.CAN-24-1938},
url = {https://inrepo02.dkfz.de/record/307373},
}