%0 Journal Article
%A Wansch, Katharina
%A Schneider, François
%A Dölvers, Florian
%A Kühn, Anna
%A Dragomir, Mihnea P
%A Joosten, Maria
%A Hilfenhaus, Georg
%A Vecchione, Loredana
%A Felsenstein, Matthäus
%A Lerchbaumer, Markus
%A Jürgensen, Christian
%A Bahra, Marcus
%A Duwe, Gregor
%A Schäfer, Reinhold
%A Stintzing, Sebastian
%A Keilholz, Ulrich
%A Neumann, Christopher C M
%A Pelzer, Uwe
%T Identifying Factors of Organoid Establishment in Pancreatic Cancer: A Prospective Observational Study.
%J Cancer medicine
%V 15
%N 1
%@ 2045-7634
%C Hoboken, NJ
%I Wiley
%M DKFZ-2026-00001
%P e71490
%D 2026
%Z #DKTKZFB26#
%X Patient-derived organoid (PDO) models have emerged as critical tools in pancreatic ductal adenocarcinoma (PDAC) research and are used as surrogates for studying the individual's tumor biology. Still, PDO-based concepts for direct clinical application remain challenging. In this prospective observational trial (OrgaPanCCC-01), we aim to address clinical feasibility, identify predictive factors for PDO establishment, and assess the prognostic potential of PDO establishment for patient's survival.Samples for PDO generation were prospectively collected via endoscopy, surgery, and transcutaneous punch biopsy, or from ascites. Patients were followed up for a median time of 14.6 months. We evaluated the clinical feasibility by determining the PDO establishment rate and the time required for establishment. Uni- and multivariate analyses were performed to examine the effect of clinical and sample characteristics on PDO establishment. For the predictive and prognostic potential, PDO establishment was correlated to the patients' disease-free (DFS), progression-free (PFS) and overall survival (OS).Between 2021 and 2023, 75 patients were enrolled with radiologically suspected PDAC at the Charité Universitätsmedizin Berlin and at the Waldfriede Krankenhaus Berlin. PDAC was confirmed in 62 patients (83
%K Humans
%K Pancreatic Neoplasms: pathology
%K Pancreatic Neoplasms: mortality
%K Pancreatic Neoplasms: therapy
%K Male
%K Female
%K Organoids: pathology
%K Prospective Studies
%K Middle Aged
%K Aged
%K Carcinoma, Pancreatic Ductal: pathology
%K Carcinoma, Pancreatic Ductal: mortality
%K Prognosis
%K Feasibility Studies
%K Adult
%K Aged, 80 and over
%K functional precision medicine (Other)
%K pancreatic ductal adenocarcinoma (Other)
%K patient‐derived organoids (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41469727
%2 pmc:PMC12753581
%R 10.1002/cam4.71490
%U https://inrepo02.dkfz.de/record/307496