%0 Journal Article
%A Zaghla, Badeel Kh Q
%A Safyürek, Zuhal
%A Gröger, Daniela
%A Mecklenbräuker, Shima
%A Lingstädt, Nina-Sophie
%A Popp, Oliver
%A Spengler, Johanna
%A Haji, Mohamed
%A Montesion, Meagan
%A Albacker, Lee A
%A Mertins, Philipp
%A Klatt, Martin Gunther
%T Systematic evaluation of neoepitope predictions challenges clinically observed T-cell responses and their impact on immune evasion.
%J Journal for ImmunoTherapy of Cancer
%V 14
%N 1
%@ 2051-1426
%C London
%I BioMed Central
%M DKFZ-2026-00071
%P e013271
%D 2026
%Z #DKTKZFB26#
%X Peptide presentation on human leukocyte antigens (HLAs) is essential for initiating T-cell responses and all consequences of this presentation including anticancer immunity or immune escape. Many studies have relied on in silico prediction tools rather than biological measurement of HLA presentation to study these effects. To better assess the frequency and consequences of neoantigen presentation, we overexpressed 125 combinations of full-length neoantigens and one HLA class I allele to experimentally validate presentation of mutated and non-mutated HLA ligands through HLA ligand isolation followed by tandem mass spectrometry. A successful presentation was observed only in 22
%K Humans
%K Immune Evasion
%K T-Lymphocytes: immunology
%K Epitopes, T-Lymphocyte: immunology
%K Antigens, Neoplasm: immunology
%K Antigens, Neoplasm: genetics
%K Antigen Presentation: immunology
%K HLA Antigens: immunology
%K HLA Antigens: genetics
%K Cancer Vaccines: immunology
%K Histocompatibility Antigens Class I: immunology
%K Histocompatibility Antigens Class I: genetics
%K Human leukocyte antigen - HLA (Other)
%K Immunotherapy (Other)
%K T cell Receptor - TCR (Other)
%K Vaccine (Other)
%K Epitopes, T-Lymphocyte (NLM Chemicals)
%K Antigens, Neoplasm (NLM Chemicals)
%K HLA Antigens (NLM Chemicals)
%K Cancer Vaccines (NLM Chemicals)
%K Histocompatibility Antigens Class I (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41506790
%R 10.1136/jitc-2025-013271
%U https://inrepo02.dkfz.de/record/307576