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@ARTICLE{Rechberger:308496,
      author       = {J. S. Rechberger and W. J. F. Vanbilloen and L. F.
                      Nonnenbroich$^*$ and J. Ge and R. S. Schrecengost and R. A.
                      Vaubel and L. Zhang and D. J. Daniels},
      title        = {{R}adiotherapy plus neoadjuvant and concomitant
                      {IL}-13{R}α2-directed immunotoxin therapy for diffuse
                      intrinsic pontine glioma.},
      journal      = {Communications biology},
      volume       = {9},
      number       = {1},
      issn         = {2399-3642},
      address      = {London},
      publisher    = {Springer Nature},
      reportid     = {DKFZ-2026-00136},
      pages        = {78},
      year         = {2026},
      note         = {#DKTKZFB26# / 2026 Jan 15;9(1):78},
      abstract     = {Radiotherapy (RT) is the standard-of-care for diffuse
                      intrinsic pontine glioma (DIPG); however, it functions as a
                      palliative treatment. Interleukin 13 receptor subunit alpha
                      2 (IL-13Rα2) is upregulated in most DIPG tumors, posing a
                      promising therapeutic target. Immunotherapies harnessing
                      IL-13Rα2 to selectively deliver cytotoxic payloads such as
                      pseudomonas exotoxin A (PE) are safe in DIPG patients and
                      efficacious in preclinical disease models. Here, we used
                      DIPG cell lines and mouse models to compare RT alone with RT
                      plus the IL-13Rα2-targeted PE immunotoxin GB13
                      (IL13.E13K-PE4E). DNA strand breaks were evaluated by γH2AX
                      and apoptosis, as well as other on-target effects, by
                      Western blot and immunofluorescence. Cell viability and
                      colony formation assays delineated cell viability and
                      proliferation. In vivo efficacy was based on survival of
                      mice with orthotopic tumors. Animals received fractionated
                      focal irradiation and neoadjuvant and concomitant GB13 by
                      convection-enhanced delivery. GB13 improved the efficacy of
                      RT in vitro through inhibition of DNA damage repair and
                      convergent modulation of apoptotic signaling. Combined RT
                      and intratumoral administration of GB13 decreased tumor
                      burden and prolonged survival in orthotopic xenograft and
                      genetically engineered mouse models. These findings indicate
                      that RT plus GB13 is well tolerated and effective, informing
                      future investigation of a novel therapeutic approach for
                      DIPG.},
      cin          = {B310 / HD01},
      ddc          = {570},
      cid          = {I:(DE-He78)B310-20160331 / I:(DE-He78)HD01-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41540212},
      doi          = {10.1038/s42003-025-09155-9},
      url          = {https://inrepo02.dkfz.de/record/308496},
}