Home > Publications database > Colonic spatial single-cell proteomics and murine models link mitochondrial dysfunction to dimeric IgA-secreting plasma cell deficiency in Crohn's disease. > print

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024 7 _ |a 10.1038/s41467-026-69069-w
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082 _ _ |a 500
100 1 _ |a Raschdorf, Annika
|0 0009-0005-7492-4307
|b 0
245 _ _ |a Colonic spatial single-cell proteomics and murine models link mitochondrial dysfunction to dimeric IgA-secreting plasma cell deficiency in Crohn's disease.
260 _ _ |a [London]
|c 2026
|b Springer Nature
336 7 _ |a article
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520 _ _ |a Secretory IgA (SIgA) is critical for maintaining the intestinal barrier. A dysregulated B-cell compartment and altered Ig secretion have been well documented in Crohn's disease (CD) patients, although their origin is unknown. To unravel the role of mucosal humoral immunity in CD pathogenesis, we in-depth phenotype colonic plasma cell (PC) differentiation in CD at the single-cell level, linked to ex vivo functional characterization and experimental mouse models with a congenital mitochondrial defect or under glucose-free high-protein dietary intervention. Here, we demonstrate that despite expanded colonic B cells, CD patients in remission present significantly diminished mucosal dimeric IgA and fecal SIgA. Colonic plasmablasts and immature CD19+CD45+ PCs are increased at the expense of the mature CD19-CD45- phenotype. Accordingly, CD-derived ex vivo differentiated PCs display impaired maturation into dimeric IgA-secreting PCs. In this study, patient-derived data from colonic RNA-seq, spatial single-cell proteomics, and plasma metabolomics are combined with data from both mouse models and highlight the crucial role of mitochondrial oxidative phosphorylation in colonic IgA+-PC differentiation, suggesting promising directions for future therapeutic strategies.
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650 _ 7 |a Immunoglobulin A, Secretory
|2 NLM Chemicals
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Crohn Disease: immunology
|2 MeSH
650 _ 2 |a Crohn Disease: metabolism
|2 MeSH
650 _ 2 |a Crohn Disease: pathology
|2 MeSH
650 _ 2 |a Plasma Cells: metabolism
|2 MeSH
650 _ 2 |a Plasma Cells: immunology
|2 MeSH
650 _ 2 |a Plasma Cells: pathology
|2 MeSH
650 _ 2 |a Colon: immunology
|2 MeSH
650 _ 2 |a Colon: pathology
|2 MeSH
650 _ 2 |a Colon: metabolism
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Mitochondria: metabolism
|2 MeSH
650 _ 2 |a Mitochondria: pathology
|2 MeSH
650 _ 2 |a Disease Models, Animal
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Proteomics: methods
|2 MeSH
650 _ 2 |a Single-Cell Analysis
|2 MeSH
650 _ 2 |a Immunoglobulin A, Secretory: metabolism
|2 MeSH
650 _ 2 |a Immunoglobulin A, Secretory: immunology
|2 MeSH
650 _ 2 |a Male
|2 MeSH
650 _ 2 |a Female
|2 MeSH
650 _ 2 |a Intestinal Mucosa: immunology
|2 MeSH
650 _ 2 |a Intestinal Mucosa: metabolism
|2 MeSH
650 _ 2 |a Intestinal Mucosa: pathology
|2 MeSH
650 _ 2 |a Mice, Inbred C57BL
|2 MeSH
650 _ 2 |a Cell Differentiation
|2 MeSH
650 _ 2 |a B-Lymphocytes: immunology
|2 MeSH
650 _ 2 |a B-Lymphocytes: metabolism
|2 MeSH
650 _ 2 |a Oxidative Phosphorylation
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700 1 _ |a de Almeida, Larissa Nogueira
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700 1 _ |a Solbach, Philipp
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700 1 _ |a Kirstein, Martha M
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700 1 _ |a Marquardt, Jens U
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700 1 _ |a Schmelter, Franziska
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700 1 _ |a Günther, Ulrich L
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700 1 _ |a Schlichting, Heidi
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700 1 _ |a Hicken, Maren
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700 1 _ |a Christiansen, Lea
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700 1 _ |a Wiestler, Miriam
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700 1 _ |a Tews, Hauke C
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700 1 _ |a Bettenworth, Dominik
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700 1 _ |a Sauer, Thorben
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700 1 _ |a Gemoll, Timo
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700 1 _ |a Ehlers, Marc
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700 1 _ |a Rosenstiel, Philip
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700 1 _ |a Manz, Rudolf A
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700 1 _ |a Künstner, Axel
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700 1 _ |a Busch, Hauke
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700 1 _ |a Sina, Christian
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700 1 _ |a Derer, Stefanie
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700 1 _ |a Katalinic, Alexander
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700 1 _ |a Oberländer, Martina
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700 1 _ |a Deck, Ruth
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700 1 _ |a Sültmann, Holger
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700 1 _ |a von Bubnoff, Nikolas
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700 1 _ |a Hutzenlaub, Tobias
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700 1 _ |a Jülg, Peter
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700 1 _ |a Burziwoda, Dominik
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773 _ _ |a 10.1038/s41467-026-69069-w
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