000119340 001__ 119340
000119340 005__ 20240228145433.0
000119340 0247_ $$2doi$$a10.1002/anie.201610292
000119340 0247_ $$2pmid$$apmid:27981706
000119340 0247_ $$2ISSN$$a0044-8249
000119340 0247_ $$2ISSN$$a0570-0833
000119340 0247_ $$2ISSN$$a1433-7851
000119340 0247_ $$2ISSN$$a1521-3773
000119340 0247_ $$2altmetric$$aaltmetric:14836323
000119340 037__ $$aDKFZ-2017-00095
000119340 041__ $$aeng
000119340 082__ $$a540
000119340 1001_ $$aKonjik, Valentino$$b0
000119340 245__ $$aThe Crystal Structure of RosB: Insights into the Reaction Mechanism of the First Member of a Family of Flavodoxin-like Enzymes.
000119340 260__ $$aWeinheim$$bWiley-VCH$$c2017
000119340 3367_ $$2DRIVER$$aarticle
000119340 3367_ $$2DataCite$$aOutput Types/Journal article
000119340 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1511333212_28941
000119340 3367_ $$2BibTeX$$aARTICLE
000119340 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000119340 3367_ $$00$$2EndNote$$aJournal Article
000119340 520__ $$a8-demethyl-8-aminoriboflavin-5'-phosphate (AFP) synthase (RosB) catalyzes the key reaction of roseoflavin biosynthesis by forming AFP from riboflavin-5'-phosphate (RP) and glutamate via the intermediates 8-demethyl-8-formylriboflavin-5'-phosphate (OHC-RP) and 8-demethyl-8-carboxylriboflavin-5'-phosphate (HO2 C-RP). To understand this reaction in which a methyl substituent of an aromatic ring is replaced by an amine we structurally characterized RosB in complex with OHC-RP (2.0 Å) and AFP (1.7 Å). RosB is composed of four flavodoxin-like subunits which have been upgraded with specific extensions and a unique C-terminal arm. It appears that RosB has evolved from an electron- or hydride-transferring flavoprotein to a sophisticated multi-step enzyme which uses RP as a substrate (and not as a cofactor). Structure-based active site analysis was complemented by mutational and isotope-based mass-spectrometric data to propose an enzymatic mechanism on an atomic basis.
000119340 536__ $$0G:(DE-HGF)POF3-317$$a317 - Translational cancer research (POF3-317)$$cPOF3-317$$fPOF III$$x0
000119340 588__ $$aDataset connected to CrossRef, PubMed,
000119340 7001_ $$aBrünle, Steffen$$b1
000119340 7001_ $$aDemmer, Ulrike$$b2
000119340 7001_ $$aVanselow, Amanda$$b3
000119340 7001_ $$0P:(DE-He78)a928ded2085c8911822370cad0b4a728$$aSandhoff, Roger$$b4$$udkfz
000119340 7001_ $$aErmler, Ulrich$$b5
000119340 7001_ $$0http://orcid.org/0000-0002-7753-2422$$aMack, Matthias$$b6
000119340 773__ $$0PERI:(DE-600)2011836-3$$a10.1002/anie.201610292$$gVol. 56, no. 4, p. 1146 - 1151$$n4$$p1146 - 1151$$tAngewandte Chemie / International edition$$v56$$x1433-7851$$y2017
000119340 909CO $$ooai:inrepo02.dkfz.de:119340$$pVDB
000119340 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000119340 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bANGEW CHEM INT EDIT : 2015
000119340 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000119340 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000119340 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000119340 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000119340 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000119340 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000119340 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000119340 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000119340 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences
000119340 915__ $$0StatID:(DE-HGF)9910$$2StatID$$aIF >= 10$$bANGEW CHEM INT EDIT : 2015
000119340 9141_ $$y2017
000119340 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)a928ded2085c8911822370cad0b4a728$$aDeutsches Krebsforschungszentrum$$b4$$kDKFZ
000119340 9131_ $$0G:(DE-HGF)POF3-317$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vTranslational cancer research$$x0
000119340 9201_ $$0I:(DE-He78)G131-20160331$$kG131$$lAG Lipid-Pathobiochemie$$x0
000119340 980__ $$ajournal
000119340 980__ $$aVDB
000119340 980__ $$aI:(DE-He78)G131-20160331
000119340 980__ $$aUNRESTRICTED