Journal Article

DKFZ-2017-00717

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Prognostic significance of clinical, histopathological, and molecular characteristics of medulloblastomas in the prospective HIT2000 multicenter clinical trial cohort.

Pietsch, T. ; Schmidt, R. ; Remke, M. ; Korshunov, A.DKFZ* ; Hovestadt, V.DKFZ* ; Jones, D.DKFZ* ; Felsberg, J.DKFZ* ; Kaulich, K.DKFZ* ; Goschzik, T. ; Kool, M.DKFZ* ; Northcott, P. A.DKFZ* ; von Hoff, K. ; von Bueren, A. O. ; Friedrich, C. ; Mynarek, M. ; Skladny, H. ; Fleischhack, G. ; Taylor, M. D. ; Cremer, F. ; Lichter, P.DKFZ* ; Faldum, A. ; Reifenberger, G.DKFZ* ; Rutkowski, S. ; Pfister, S. (Last author)DKFZ*

2014
Springer Berlin

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Please use a persistent id in citations: doi:10.1007/s00401-014-1276-0

Abstract: This study aimed to prospectively evaluate clinical, histopathological and molecular variables for outcome prediction in medulloblastoma patients. Patients from the HIT2000 cooperative clinical trial were prospectively enrolled based on the availability of sufficient tumor material and complete clinical information. This revealed a cohort of 184 patients (median age 7.6 years), which was randomly split at a 2:1 ratio into a training (n = 127), and a test (n = 57) dataset in order to build and test a risk score for this population. Independent validation was performed in a non-overlapping cohort (n = 83). All samples were subjected to thorough histopathological investigation, CTNNB1 mutation analysis, quantitative PCR, MLPA and FISH analyses for cytogenetic variables, and methylome analysis. By univariable analysis, clinical factors (M-stage), histopathological variables (large cell component, endothelial proliferation, synaptophysin pattern), and molecular features (chromosome 6q status, MYC amplification, subgrouping) were found to be prognostic. Molecular consensus subgrouping (WNT, SHH, Group 3, Group 4) was validated as an independent feature to stratify patients into different risk groups. When comparing methods for the identification of WNT-driven medulloblastoma, this study identified CTNNB1 sequencing and methylation profiling to most reliably identify these patients. After removing patients with particularly favorable (CTNNB1 mutation, extensive nodularity) or unfavorable (MYC amplification) markers, a risk score for the remaining 'intermediate molecular risk' population dependent on age, M-stage, pattern of synaptophysin expression, and MYCN copy-number status was identified, with speckled synaptophysin expression indicating worse outcome. Test and independent validation of the score confirmed significant discrimination of patients by risk profile. Methylation subgrouping and CTNNB1 mutation status represent robust tools for the risk stratification of medulloblastoma. A simple clinico-pathological risk score was identified, which was confirmed in a test set and by independent clinical validation.

Keyword(s): Biomarkers ; CTNNB1 protein, human ; MYCN protein, human ; N-Myc Proto-Oncogene Protein ; Nuclear Proteins ; Oncogene Proteins ; SYP protein, human ; Synaptophysin ; beta Catenin

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Contributing Institute(s):

1. KKE Neuropathologie (G380)
2. Molekulare Genetik (B060)
3. Pädiatrische Neuroonkologie (B062)
4. DKTK Heidelberg (L101)
5. DKTK Essen (L401)

Research Program(s):

1. 312 - Functional and structural genomics (POF3-312) (POF3-312)

Appears in the scientific report 2014

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Database coverage:
; BIOSIS Previews ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 10 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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