TY  - JOUR
AU  - Aleksandrowicz, Ewa
AU  - Herr, Ingrid
TI  - Ethical euthanasia and short-term anesthesia of the chick embryo.
JO  - Alternatives to animal experimentation
VL  - 32
IS  - 2
SN  - 1868-596X
CY  - Heidelberg
PB  - ALTEX Edition
M1  - DKFZ-2017-02145
SP  - 143-147
PY  - 2015
AB  - Fertilized chicken eggs are suggested as an alternative to mammalian models. The chorioallantoic membrane (CAM) of the chick embryo is widely used for examination of angiogenesis, xenotransplants and for virus production. Unfortunately, it is mostly not taken into account, that the chick embryo's ability to experience pain starts to develop at day 7 of breeding. In our view, this model is only in accordance with the 3 R principles, if an appropriate anesthesia of the chick embryo in potentially painful procedures is provided. Although many experimental approaches are performed on the none-innervated CAM, the euthanasia of the embryo strongly requires a more human technique than the usually used freezing at -20°C, decapitation or in ovo fixation with paraformaldehyde without prior anesthesia. However, protocols regarding feasible and ethical methods for anesthesia and euthanasia of avian embryos are currently not available. Therefore, we established an easy and reliable method for the euthanasia and short-term anesthesia of the chick embryo.
LB  - PUB:(DE-HGF)16
C6  - pmid:25592390
DO  - DOI:10.14573/altex.1410031
UR  - https://inrepo02.dkfz.de/record/126030
ER  -