Journal Article

DKFZ-2017-02145

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Ethical euthanasia and short-term anesthesia of the chick embryo.

Aleksandrowicz, E. (First author)DKFZ* ; Herr, I. (Last author)DKFZ*

2015
ALTEX Edition Heidelberg

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Please use a persistent id in citations: doi:10.14573/altex.1410031

Abstract: Fertilized chicken eggs are suggested as an alternative to mammalian models. The chorioallantoic membrane (CAM) of the chick embryo is widely used for examination of angiogenesis, xenotransplants and for virus production. Unfortunately, it is mostly not taken into account, that the chick embryo's ability to experience pain starts to develop at day 7 of breeding. In our view, this model is only in accordance with the 3 R principles, if an appropriate anesthesia of the chick embryo in potentially painful procedures is provided. Although many experimental approaches are performed on the none-innervated CAM, the euthanasia of the embryo strongly requires a more human technique than the usually used freezing at -20°C, decapitation or in ovo fixation with paraformaldehyde without prior anesthesia. However, protocols regarding feasible and ethical methods for anesthesia and euthanasia of avian embryos are currently not available. Therefore, we established an easy and reliable method for the euthanasia and short-term anesthesia of the chick embryo.

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Contributing Institute(s):

1. Molekulare Onkochirurgie (G403)

Research Program(s):

1. 317 - Translational cancer research (POF3-317) (POF3-317)

Appears in the scientific report 2015

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Database coverage:
; ; DOAJ Seal ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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