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@ARTICLE{Aleksandrowicz:126030,
      author       = {E. Aleksandrowicz$^*$ and I. Herr$^*$},
      title        = {{E}thical euthanasia and short-term anesthesia of the chick
                      embryo.},
      journal      = {Alternatives to animal experimentation},
      volume       = {32},
      number       = {2},
      issn         = {1868-596X},
      address      = {Heidelberg},
      publisher    = {ALTEX Edition},
      reportid     = {DKFZ-2017-02145},
      pages        = {143-147},
      year         = {2015},
      abstract     = {Fertilized chicken eggs are suggested as an alternative to
                      mammalian models. The chorioallantoic membrane (CAM) of the
                      chick embryo is widely used for examination of angiogenesis,
                      xenotransplants and for virus production. Unfortunately, it
                      is mostly not taken into account, that the chick embryo's
                      ability to experience pain starts to develop at day 7 of
                      breeding. In our view, this model is only in accordance with
                      the 3 R principles, if an appropriate anesthesia of the
                      chick embryo in potentially painful procedures is provided.
                      Although many experimental approaches are performed on the
                      none-innervated CAM, the euthanasia of the embryo strongly
                      requires a more human technique than the usually used
                      freezing at -20°C, decapitation or in ovo fixation with
                      paraformaldehyde without prior anesthesia. However,
                      protocols regarding feasible and ethical methods for
                      anesthesia and euthanasia of avian embryos are currently not
                      available. Therefore, we established an easy and reliable
                      method for the euthanasia and short-term anesthesia of the
                      chick embryo.},
      cin          = {G403},
      ddc          = {610},
      cid          = {I:(DE-He78)G403-20160331},
      pnm          = {317 - Translational cancer research (POF3-317)},
      pid          = {G:(DE-HGF)POF3-317},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:25592390},
      doi          = {10.14573/altex.1410031},
      url          = {https://inrepo02.dkfz.de/record/126030},
}