%0 Journal Article
%A Kahlert, C.
%A Pecqueux, M.
%A Halama, Niels
%A Dienemann, H.
%A Muley, T.
%A Pfannschmidt, J.
%A Lasitschka, F.
%A Klupp, F.
%A Schmidt, T.
%A Rahbari, N.
%A Reissfelder, C.
%A Kunz, Christina
%A Benner, Axel
%A Falk, C.
%A Weitz, J.
%A Koch, M.
%T Tumour-site-dependent expression profile of angiogenic factors in tumour-associated stroma of primary colorectal cancer and metastases.
%J British journal of cancer
%V 110
%N 2
%@ 1532-1827
%C Edinburgh
%I Nature Publ. Group
%M DKFZ-2017-02743
%P 441 - 449
%D 2014
%X Tumour-associated stroma has a critical role in tumour proliferation. Our aim was to determine a specific protein expression profile of stromal angiogenic cytokines and matrix metalloproteinases (MMPs) to identify potential biomarkers or new therapy targets.Frozen tissue of primary colorectal cancer (n=25), liver (n=25) and lung metastases (n=23) was laser-microdissected to obtain tumour epithelial cells and adjacent tumour-associated stroma. Protein expression of nine angiogenic cytokines and eight MMPs was analysed using a multiplex-based protein assay.We found a differential expression of several MMPs and angiogenic cytokines in tumour cells compared with adjacent tumour stroma. Cluster analysis displayed a tumour-site-dependent stromal expression of MMPs and angiogenic cytokines. Univariate analysis identified stromal MMP-2 and MMP-3 in primary colorectal cancer, stromal MMP-1, -2, -3 and Angiopoietin-2 in lung metastases and stromal MMP-12 and VEGF in liver metastases as prognostic markers (P>0.05, respectively). Furthermore, stroma-derived Angiopoietin-2 proved to be an independent prognostic marker in colorectal lung metastases.Expression of MMPs and angiogenic cytokines in tumour cells and adjacent tumour stroma is dependent on the tumour site. Stroma-derived MMPs and angiogenic cytokines may be useful prognostic biomarkers. These data can be helpful to identify new agents for a targeted therapy in patients with colorectal cancer.
%K Angiogenesis Inducing Agents (NLM Chemicals)
%K Angiopoietin-2 (NLM Chemicals)
%K Biomarkers, Tumor (NLM Chemicals)
%K Cytokines (NLM Chemicals)
%K VEGFA protein, human (NLM Chemicals)
%K Vascular Endothelial Growth Factor A (NLM Chemicals)
%K Matrix Metalloproteinases (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:24292449
%2 pmc:PMC3899771
%R 10.1038/bjc.2013.745
%U https://inrepo02.dkfz.de/record/126715