Journal Article

DKFZ-2017-02743

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Tumour-site-dependent expression profile of angiogenic factors in tumour-associated stroma of primary colorectal cancer and metastases.

Kahlert, C. ; Pecqueux, M. ; Halama, N.DKFZ* ; Dienemann, H. ; Muley, T. ; Pfannschmidt, J. ; Lasitschka, F. ; Klupp, F. ; Schmidt, T. ; Rahbari, N. ; Reissfelder, C. ; Kunz, C.DKFZ* ; Benner, A.DKFZ* ; Falk, C. ; Weitz, J. ; Koch, M.

2014
Nature Publ. Group Edinburgh

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Please use a persistent id in citations: doi:10.1038/bjc.2013.745

Abstract: Tumour-associated stroma has a critical role in tumour proliferation. Our aim was to determine a specific protein expression profile of stromal angiogenic cytokines and matrix metalloproteinases (MMPs) to identify potential biomarkers or new therapy targets.Frozen tissue of primary colorectal cancer (n=25), liver (n=25) and lung metastases (n=23) was laser-microdissected to obtain tumour epithelial cells and adjacent tumour-associated stroma. Protein expression of nine angiogenic cytokines and eight MMPs was analysed using a multiplex-based protein assay.We found a differential expression of several MMPs and angiogenic cytokines in tumour cells compared with adjacent tumour stroma. Cluster analysis displayed a tumour-site-dependent stromal expression of MMPs and angiogenic cytokines. Univariate analysis identified stromal MMP-2 and MMP-3 in primary colorectal cancer, stromal MMP-1, -2, -3 and Angiopoietin-2 in lung metastases and stromal MMP-12 and VEGF in liver metastases as prognostic markers (P>0.05, respectively). Furthermore, stroma-derived Angiopoietin-2 proved to be an independent prognostic marker in colorectal lung metastases.Expression of MMPs and angiogenic cytokines in tumour cells and adjacent tumour stroma is dependent on the tumour site. Stroma-derived MMPs and angiogenic cytokines may be useful prognostic biomarkers. These data can be helpful to identify new agents for a targeted therapy in patients with colorectal cancer.

Keyword(s): Angiogenesis Inducing Agents ; Angiopoietin-2 ; Biomarkers, Tumor ; Cytokines ; VEGFA protein, human ; Vascular Endothelial Growth Factor A ; Matrix Metalloproteinases

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Contributing Institute(s):

1. Biostatistik (C060)
2. Angewandte Tumor-Immunität (D120)

Research Program(s):

1. 313 - Cancer risk factors and prevention (POF3-313) (POF3-313)

Appears in the scientific report 2014

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Database coverage:
; BIOSIS Previews ; Current Contents - Clinical Medicine ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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