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@ARTICLE{Kahlert:126715,
      author       = {C. Kahlert and M. Pecqueux and N. Halama$^*$ and H.
                      Dienemann and T. Muley and J. Pfannschmidt and F. Lasitschka
                      and F. Klupp and T. Schmidt and N. Rahbari and C.
                      Reissfelder and C. Kunz$^*$ and A. Benner$^*$ and C. Falk
                      and J. Weitz and M. Koch},
      title        = {{T}umour-site-dependent expression profile of angiogenic
                      factors in tumour-associated stroma of primary colorectal
                      cancer and metastases.},
      journal      = {British journal of cancer},
      volume       = {110},
      number       = {2},
      issn         = {1532-1827},
      address      = {Edinburgh},
      publisher    = {Nature Publ. Group},
      reportid     = {DKFZ-2017-02743},
      pages        = {441 - 449},
      year         = {2014},
      abstract     = {Tumour-associated stroma has a critical role in tumour
                      proliferation. Our aim was to determine a specific protein
                      expression profile of stromal angiogenic cytokines and
                      matrix metalloproteinases (MMPs) to identify potential
                      biomarkers or new therapy targets.Frozen tissue of primary
                      colorectal cancer (n=25), liver (n=25) and lung metastases
                      (n=23) was laser-microdissected to obtain tumour epithelial
                      cells and adjacent tumour-associated stroma. Protein
                      expression of nine angiogenic cytokines and eight MMPs was
                      analysed using a multiplex-based protein assay.We found a
                      differential expression of several MMPs and angiogenic
                      cytokines in tumour cells compared with adjacent tumour
                      stroma. Cluster analysis displayed a tumour-site-dependent
                      stromal expression of MMPs and angiogenic cytokines.
                      Univariate analysis identified stromal MMP-2 and MMP-3 in
                      primary colorectal cancer, stromal MMP-1, -2, -3 and
                      Angiopoietin-2 in lung metastases and stromal MMP-12 and
                      VEGF in liver metastases as prognostic markers (P>0.05,
                      respectively). Furthermore, stroma-derived Angiopoietin-2
                      proved to be an independent prognostic marker in colorectal
                      lung metastases.Expression of MMPs and angiogenic cytokines
                      in tumour cells and adjacent tumour stroma is dependent on
                      the tumour site. Stroma-derived MMPs and angiogenic
                      cytokines may be useful prognostic biomarkers. These data
                      can be helpful to identify new agents for a targeted therapy
                      in patients with colorectal cancer.},
      keywords     = {Angiogenesis Inducing Agents (NLM Chemicals) /
                      Angiopoietin-2 (NLM Chemicals) / Biomarkers, Tumor (NLM
                      Chemicals) / Cytokines (NLM Chemicals) / VEGFA protein,
                      human (NLM Chemicals) / Vascular Endothelial Growth Factor A
                      (NLM Chemicals) / Matrix Metalloproteinases (NLM Chemicals)},
      cin          = {C060 / D120},
      ddc          = {610},
      cid          = {I:(DE-He78)C060-20160331 / I:(DE-He78)D120-20160331},
      pnm          = {313 - Cancer risk factors and prevention (POF3-313)},
      pid          = {G:(DE-HGF)POF3-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:24292449},
      pmc          = {pmc:PMC3899771},
      doi          = {10.1038/bjc.2013.745},
      url          = {https://inrepo02.dkfz.de/record/126715},
}