%0 Journal Article
%A Meister, Michael
%A Tounsi, Amel
%A Gaffal, Evelyn
%A Bald, Tobias
%A Papatriantafyllou, Maria
%A Ludwig, Julia
%A Pougialis, Georg
%A Bestvater, Felix
%A Klotz, Luisa
%A Moldenhauer, Gerhard
%A Tüting, Thomas
%A Hämmerling, Günter
%A Arnold, Bernd
%A Oelert, Thilo
%T Self-Antigen Presentation by Keratinocytes in the Inflamed Adult Skin Modulates T-Cell Auto-Reactivity.
%J The journal of investigative dermatology
%V 135
%N 8
%@ 0022-202X
%C Amsterdam
%I Elsevier
%M DKFZ-2017-03118
%P 1996 - 2004
%D 2015
%X Keratinocytes have a pivotal role in the regulation of immune responses, but the impact of antigen presentation by these cells is still poorly understood, particularly in a situation where the antigen will be presented only in adult life. Here, we generated a transgenic mouse model in which keratinocytes exclusively present a myelin basic protein (MBP) peptide covalently linked to the major histocompatibility complex class II β-chain, solely under inflammatory conditions. In these mice, inflammation caused by epicutaneous contact sensitizer treatment resulted in keratinocyte-mediated expansion of MBP-specific CD4(+) T cells in the skin. Moreover, repeated contact sensitizer application preceding a systemic MBP immunization reduced the reactivity of the respective CD4(+) T cells and lowered the symptoms of the resulting experimental autoimmune encephalomyelitis. This downregulation was CD4(+) T-cell-mediated and dependent on the presence of the immune modulator Dickkopf-3. Thus, presentation of a neo self-antigen by keratinocytes in the inflamed, adult skin can modulate CD4(+) T-cell auto-aggression at a distal organ.
%K 4-ethoxymethylene-2-phenyl-2-oxazoline-5-one (NLM Chemicals)
%K Autoantigens (NLM Chemicals)
%K Cyclopropanes (NLM Chemicals)
%K Dkk3 protein, mouse (NLM Chemicals)
%K Intercellular Signaling Peptides and Proteins (NLM Chemicals)
%K Myelin Basic Protein (NLM Chemicals)
%K Oxazoles (NLM Chemicals)
%K diphenylcyclopropenone (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:25835957
%R 10.1038/jid.2015.130
%U https://inrepo02.dkfz.de/record/127092