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@ARTICLE{Tuorto:127657,
      author       = {F. Tuorto$^*$ and F. Herbst$^*$ and N. Alerasool$^*$ and S.
                      Bender$^*$ and O. Popp and G. Federico$^*$ and S.
                      Reitter$^*$ and R. Liebers$^*$ and G. Stoecklin$^*$ and
                      H.-J. Gröne$^*$ and G. Dittmar and H. Glimm$^*$ and F.
                      Lyko$^*$},
      title        = {{T}he t{RNA} methyltransferase {D}nmt2 is required for
                      accurate polypeptide synthesis during haematopoiesis.},
      journal      = {The EMBO journal},
      volume       = {34},
      number       = {18},
      issn         = {1460-2075},
      address      = {Heidelberg},
      publisher    = {EMBO Press},
      reportid     = {DKFZ-2017-03680},
      pages        = {2350 - 2362},
      year         = {2015},
      abstract     = {The Dnmt2 enzyme utilizes the catalytic mechanism of
                      eukaryotic DNA methyltransferases to methylate several tRNAs
                      at cytosine 38. Dnmt2 mutant mice, flies, and plants were
                      reported to be viable and fertile, and the biological
                      function of Dnmt2 has remained elusive. Here, we show that
                      endochondral ossification is delayed in newborn
                      Dnmt2-deficient mice, which is accompanied by a reduction of
                      the haematopoietic stem and progenitor cell population and a
                      cell-autonomous defect in their differentiation. RNA
                      bisulfite sequencing revealed that Dnmt2 methylates C38 of
                      tRNA Asp(GTC), Gly(GCC), and Val(AAC), thus preventing tRNA
                      fragmentation. Proteomic analyses from primary bone marrow
                      cells uncovered systematic differences in protein expression
                      that are due to specific codon mistranslation by tRNAs
                      lacking Dnmt2-dependent methylation. Our observations
                      demonstrate that Dnmt2 plays an important role in
                      haematopoiesis and define a novel function of C38 tRNA
                      methylation in the discrimination of near-cognate codons,
                      thereby ensuring accurate polypeptide synthesis.},
      keywords     = {RNA, Transfer (NLM Chemicals) / Dnmt2 protein, mouse (NLM
                      Chemicals) / DNA (Cytosine-5-)-Methyltransferase (NLM
                      Chemicals)},
      cin          = {A130 / G100 / A200 / G130},
      ddc          = {570},
      cid          = {I:(DE-He78)A130-20160331 / I:(DE-He78)G100-20160331 /
                      I:(DE-He78)A200-20160331 / I:(DE-He78)G130-20160331},
      pnm          = {311 - Signalling pathways, cell and tumor biology
                      (POF3-311)},
      pid          = {G:(DE-HGF)POF3-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:26271101},
      pmc          = {pmc:PMC4570521},
      doi          = {10.15252/embj.201591382},
      url          = {https://inrepo02.dkfz.de/record/127657},
}