guest ::
| Home > Publications database > The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis. |
| Home > Publications database > The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis. |
| Journal Article | DKFZ-2017-03680 |
; ; ; ; ; ; ; ; ; ; ; ;
2015
EMBO Press
Heidelberg
This record in other databases: 
Please use a persistent id in citations: doi:10.15252/embj.201591382
Abstract: The Dnmt2 enzyme utilizes the catalytic mechanism of eukaryotic DNA methyltransferases to methylate several tRNAs at cytosine 38. Dnmt2 mutant mice, flies, and plants were reported to be viable and fertile, and the biological function of Dnmt2 has remained elusive. Here, we show that endochondral ossification is delayed in newborn Dnmt2-deficient mice, which is accompanied by a reduction of the haematopoietic stem and progenitor cell population and a cell-autonomous defect in their differentiation. RNA bisulfite sequencing revealed that Dnmt2 methylates C38 of tRNA Asp(GTC), Gly(GCC), and Val(AAC), thus preventing tRNA fragmentation. Proteomic analyses from primary bone marrow cells uncovered systematic differences in protein expression that are due to specific codon mistranslation by tRNAs lacking Dnmt2-dependent methylation. Our observations demonstrate that Dnmt2 plays an important role in haematopoiesis and define a novel function of C38 tRNA methylation in the discrimination of near-cognate codons, thereby ensuring accurate polypeptide synthesis.
Keyword(s): RNA, Transfer ; Dnmt2 protein, mouse ; DNA (Cytosine-5-)-Methyltransferase
; BIOSIS Previews ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection
|
The record appears in these collections: |
