guest ::
| Home > Publications database > The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis. > print |
| Home > Publications database > The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis. > print |
| 001 | 127657 | ||
| 005 | 20240228140942.0 | ||
| 024 | 7 | _ | |a 10.15252/embj.201591382 |2 doi |
| 024 | 7 | _ | |a pmid:26271101 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC4570521 |2 pmc |
| 024 | 7 | _ | |a 0261-4189 |2 ISSN |
| 024 | 7 | _ | |a 1460-2075 |2 ISSN |
| 024 | 7 | _ | |a altmetric:4393988 |2 altmetric |
| 037 | _ | _ | |a DKFZ-2017-03680 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Tuorto, Francesca |0 P:(DE-He78)93d163dbfed1c057437cbe97439bba99 |b 0 |e First author |u dkfz |
| 245 | _ | _ | |a The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis. |
| 260 | _ | _ | |a Heidelberg |c 2015 |b EMBO Press |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1508330009_30693 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a The Dnmt2 enzyme utilizes the catalytic mechanism of eukaryotic DNA methyltransferases to methylate several tRNAs at cytosine 38. Dnmt2 mutant mice, flies, and plants were reported to be viable and fertile, and the biological function of Dnmt2 has remained elusive. Here, we show that endochondral ossification is delayed in newborn Dnmt2-deficient mice, which is accompanied by a reduction of the haematopoietic stem and progenitor cell population and a cell-autonomous defect in their differentiation. RNA bisulfite sequencing revealed that Dnmt2 methylates C38 of tRNA Asp(GTC), Gly(GCC), and Val(AAC), thus preventing tRNA fragmentation. Proteomic analyses from primary bone marrow cells uncovered systematic differences in protein expression that are due to specific codon mistranslation by tRNAs lacking Dnmt2-dependent methylation. Our observations demonstrate that Dnmt2 plays an important role in haematopoiesis and define a novel function of C38 tRNA methylation in the discrimination of near-cognate codons, thereby ensuring accurate polypeptide synthesis. |
| 536 | _ | _ | |a 311 - Signalling pathways, cell and tumor biology (POF3-311) |0 G:(DE-HGF)POF3-311 |c POF3-311 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a RNA, Transfer |0 9014-25-9 |2 NLM Chemicals |
| 650 | _ | 7 | |a Dnmt2 protein, mouse |0 EC 2.1.1.- |2 NLM Chemicals |
| 650 | _ | 7 | |a DNA (Cytosine-5-)-Methyltransferase |0 EC 2.1.1.37 |2 NLM Chemicals |
| 700 | 1 | _ | |a Herbst, Friederike |0 P:(DE-He78)13dc15153ce40f775007112548f34d79 |b 1 |u dkfz |
| 700 | 1 | _ | |a Alerasool, Nader |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Bender, Sebastian |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Popp, Oliver |b 4 |
| 700 | 1 | _ | |a Federico, Giuseppina |0 P:(DE-He78)616706c4d6f7bdf68b30893f860cbb2b |b 5 |u dkfz |
| 700 | 1 | _ | |a Reitter, Sonja |0 P:(DE-He78)d1bdeec6c1345e82a575125e894c5283 |b 6 |u dkfz |
| 700 | 1 | _ | |a Liebers, Reinhard |0 P:(DE-He78)0cc6dd5c419a9456e7b7d99b6888eb79 |b 7 |u dkfz |
| 700 | 1 | _ | |a Stoecklin, Georg |0 P:(DE-HGF)0 |b 8 |
| 700 | 1 | _ | |a Gröne, Hermann-Josef |0 P:(DE-He78)00a2ea610aee4a8fca32908fc3d02e91 |b 9 |u dkfz |
| 700 | 1 | _ | |a Dittmar, Gunnar |b 10 |
| 700 | 1 | _ | |a Glimm, Hanno |0 P:(DE-He78)157277fe62f07df1732f9d126a51d1b9 |b 11 |u dkfz |
| 700 | 1 | _ | |a Lyko, Frank |0 P:(DE-He78)a8d53a8cdc716390a6cbacdead227143 |b 12 |e Last author |u dkfz |
| 773 | _ | _ | |a 10.15252/embj.201591382 |g Vol. 34, no. 18, p. 2350 - 2362 |0 PERI:(DE-600)1467419-1 |n 18 |p 2350 - 2362 |t The EMBO journal |v 34 |y 2015 |x 1460-2075 |
| 909 | C | O | |o oai:inrepo02.dkfz.de:127657 |p VDB |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 0 |6 P:(DE-He78)93d163dbfed1c057437cbe97439bba99 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 1 |6 P:(DE-He78)13dc15153ce40f775007112548f34d79 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 2 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 3 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 5 |6 P:(DE-He78)616706c4d6f7bdf68b30893f860cbb2b |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 6 |6 P:(DE-He78)d1bdeec6c1345e82a575125e894c5283 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 7 |6 P:(DE-He78)0cc6dd5c419a9456e7b7d99b6888eb79 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 8 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 9 |6 P:(DE-He78)00a2ea610aee4a8fca32908fc3d02e91 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 11 |6 P:(DE-He78)157277fe62f07df1732f9d126a51d1b9 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 12 |6 P:(DE-He78)a8d53a8cdc716390a6cbacdead227143 |
| 913 | 1 | _ | |a DE-HGF |l Krebsforschung |1 G:(DE-HGF)POF3-310 |0 G:(DE-HGF)POF3-311 |2 G:(DE-HGF)POF3-300 |v Signalling pathways, cell and tumor biology |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |b Gesundheit |
| 914 | 1 | _ | |y 2015 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b EMBO J : 2015 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0600 |2 StatID |b Ebsco Academic Search |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b ASC |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |
| 915 | _ | _ | |a IF >= 5 |0 StatID:(DE-HGF)9905 |2 StatID |b EMBO J : 2015 |
| 920 | 1 | _ | |0 I:(DE-He78)A130-20160331 |k A130 |l Epigenetik |x 0 |
| 920 | 1 | _ | |0 I:(DE-He78)G100-20160331 |k G100 |l Translationale Onkologie |x 1 |
| 920 | 1 | _ | |0 I:(DE-He78)A200-20160331 |k A200 |l Posttranskriptionelle Genregulation |x 2 |
| 920 | 1 | _ | |0 I:(DE-He78)G130-20160331 |k G130 |l Zelluläre und Molekulare Pathologie |x 3 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-He78)A130-20160331 |
| 980 | _ | _ | |a I:(DE-He78)G100-20160331 |
| 980 | _ | _ | |a I:(DE-He78)A200-20160331 |
| 980 | _ | _ | |a I:(DE-He78)G130-20160331 |
| 980 | _ | _ | |a UNRESTRICTED |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|