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@ARTICLE{Wang:127746,
author = {X. Wang and A. M. Dubuc and V. Ramaswamy and S. Mack and D.
M. A. Gendoo and M. Remke and X. Wu and L. Garzia and B. Luu
and F. Cavalli and J. Peacock and B. López and P. Skowron
and D. Zagzag and D. Lyden and C. Hoffman and Y.-J. Cho and
C. Eberhart and T. MacDonald and X.-N. Li and T. Van Meter
and P. A. Northcott$^*$ and B. Haibe-Kains and C. Hawkins
and J. T. Rutka and E. Bouffet and S. Pfister$^*$ and A.
Korshunov$^*$ and M. D. Taylor},
title = {{M}edulloblastoma subgroups remain stable across primary
and metastatic compartments.},
journal = {Acta neuropathologica},
volume = {129},
number = {3},
issn = {1432-0533},
address = {Berlin},
publisher = {Springer},
reportid = {DKFZ-2017-03769},
pages = {449 - 457},
year = {2015},
abstract = {Medulloblastoma comprises four distinct molecular variants
with distinct genetics, transcriptomes, and outcomes.
Subgroup affiliation has been previously shown to remain
stable at the time of recurrence, which likely reflects
their distinct cells of origin. However, a therapeutically
relevant question that remains unanswered is subgroup
stability in the metastatic compartment. We assembled a
cohort of 12-paired primary-metastatic tumors collected in
the MAGIC consortium, and established their molecular
subgroup affiliation by performing integrative gene
expression and DNA methylation analysis. Frozen tissues were
collected and profiled using Affymetrix gene expression
arrays and Illumina methylation arrays. Class prediction and
hierarchical clustering were performed using existing
published datasets. Our molecular analysis, using consensus
integrative genomic data, establishes the unequivocal
maintenance of molecular subgroup affiliation in metastatic
medulloblastoma. We further validated these findings by
interrogating a non-overlapping cohort of 19 pairs of
primary-metastatic tumors from the Burdenko Neurosurgical
Institute using an orthogonal technique of
immunohistochemical staining. This investigation represents
the largest reported primary-metastatic paired cohort
profiled to date and provides a unique opportunity to
evaluate subgroup-specific molecular aberrations within the
metastatic compartment. Our findings further support the
hypothesis that medulloblastoma subgroups arise from
distinct cells of origin, which are carried forward from
ontogeny to oncology.},
cin = {B062 / G380 / L101},
ddc = {610},
cid = {I:(DE-He78)B062-20160331 / I:(DE-He78)G380-20160331 /
I:(DE-He78)L101-20160331},
pnm = {312 - Functional and structural genomics (POF3-312)},
pid = {G:(DE-HGF)POF3-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:25689980},
pmc = {pmc:PMC4333718},
doi = {10.1007/s00401-015-1389-0},
url = {https://inrepo02.dkfz.de/record/127746},
}