Journal Article

DKFZ-2017-03769

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Medulloblastoma subgroups remain stable across primary and metastatic compartments.

Wang, X. ; Dubuc, A. M. ; Ramaswamy, V. ; Mack, S. ; Gendoo, D. M. A. ; Remke, M. ; Wu, X. ; Garzia, L. ; Luu, B. ; Cavalli, F. ; Peacock, J. ; López, B. ; Skowron, P. ; Zagzag, D. ; Lyden, D. ; Hoffman, C. ; Cho, Y.-J. ; Eberhart, C. ; MacDonald, T. ; Li, X.-N. ; Van Meter, T. ; Northcott, P. A.DKFZ* ; Haibe-Kains, B. ; Hawkins, C. ; Rutka, J. T. ; Bouffet, E. ; Pfister, S.DKFZ* ; Korshunov, A.DKFZ* ; Taylor, M. D.

2015
Springer Berlin

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Please use a persistent id in citations: doi:10.1007/s00401-015-1389-0

Abstract: Medulloblastoma comprises four distinct molecular variants with distinct genetics, transcriptomes, and outcomes. Subgroup affiliation has been previously shown to remain stable at the time of recurrence, which likely reflects their distinct cells of origin. However, a therapeutically relevant question that remains unanswered is subgroup stability in the metastatic compartment. We assembled a cohort of 12-paired primary-metastatic tumors collected in the MAGIC consortium, and established their molecular subgroup affiliation by performing integrative gene expression and DNA methylation analysis. Frozen tissues were collected and profiled using Affymetrix gene expression arrays and Illumina methylation arrays. Class prediction and hierarchical clustering were performed using existing published datasets. Our molecular analysis, using consensus integrative genomic data, establishes the unequivocal maintenance of molecular subgroup affiliation in metastatic medulloblastoma. We further validated these findings by interrogating a non-overlapping cohort of 19 pairs of primary-metastatic tumors from the Burdenko Neurosurgical Institute using an orthogonal technique of immunohistochemical staining. This investigation represents the largest reported primary-metastatic paired cohort profiled to date and provides a unique opportunity to evaluate subgroup-specific molecular aberrations within the metastatic compartment. Our findings further support the hypothesis that medulloblastoma subgroups arise from distinct cells of origin, which are carried forward from ontogeny to oncology.

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Contributing Institute(s):

1. Pädiatrische Neuroonkologie (B062)
2. KKE Neuropathologie (G380)
3. DKTK Heidelberg (L101)

Research Program(s):

1. 312 - Functional and structural genomics (POF3-312) (POF3-312)

Appears in the scientific report 2015

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Database coverage:
; BIOSIS Previews ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 10 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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