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@ARTICLE{Butkevich:128616,
author = {A. N. Butkevich and V. N. Belov and K. Kolmakov and V. V.
Sokolov and H. Shojaei and S. C. Sidenstein and D. Kamin and
J. Matthias$^*$ and R. Vlijm$^*$ and J. Engelhardt$^*$ and
S. W. Hell$^*$},
title = {{H}ydroxylated {F}luorescent {D}yes for {L}ive-{C}ell
{L}abeling: {S}ynthesis, {S}pectra and {S}uper-{R}esolution
{STED}.},
journal = {Chemistry - a European journal},
volume = {23},
number = {50},
issn = {0947-6539},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {DKFZ-2017-04632},
pages = {12114 - 12119},
year = {2017},
abstract = {Hydroxylated rhodamines, carbopyronines, silico- and
germanorhodamines with absorption maxima in the range of
530-640 nm were prepared and applied in specific labeling
of living cells. The direct and high-yielding entry to
germa- and silaxanthones tolerates the presence of protected
heteroatoms and may be considered for the syntheses of
various sila- and germafluoresceins, as well as -rhodols.
Application in stimulated emission depletion (STED)
fluorescence microscopy revealed a resolution of 50-75 nm
in one- and two-color imaging of vimentin-HaloTag fused
protein and native tubulin. The established
structure-property relationships allow for prediction of the
spectral properties and the positions of
spirolactone/zwitterion equilibria for the new analogues of
rhodamines, carbo-, silico-, and germanorhodamines using
simple additive schemes.},
cin = {E190},
ddc = {540},
cid = {I:(DE-He78)E190-20160331},
pnm = {315 - Imaging and radiooncology (POF3-315)},
pid = {G:(DE-HGF)POF3-315},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28370443},
pmc = {pmc:PMC5599963},
doi = {10.1002/chem.201701216},
url = {https://inrepo02.dkfz.de/record/128616},
}