Journal Article

DKFZ-2017-04632

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED.

Butkevich, A. N. ; Belov, V. N. ; Kolmakov, K. ; Sokolov, V. V. ; Shojaei, H. ; Sidenstein, S. C. ; Kamin, D. ; Matthias, J.DKFZ* ; Vlijm, R.DKFZ* ; Engelhardt, J.DKFZ* ; Hell, S. W. (Last author)DKFZ*

2017
Wiley-VCH Weinheim

This record in other databases:  

Please use a persistent id in citations: doi:10.1002/chem.201701216

Abstract: Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50-75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure-property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes.

---

Contributing Institute(s):

1. Optische Nanoskopie (E190)

Research Program(s):

1. 315 - Imaging and radiooncology (POF3-315) (POF3-315)

Appears in the scientific report 2017

---

Database coverage:
; Current Contents - Physical, Chemical and Earth Sciences ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---