%0 Journal Article
%A Engeland, Christine
%A Bossow, Sascha
%A Hudacek, Andrew W
%A Hoyler, Birgit
%A Förster, Judith
%A Veinalde, Rūta
%A Jäger, Dirk
%A Cattaneo, Roberto
%A Ungerechts, Guy
%A Springfeld, Christoph
%T A Tupaia paramyxovirus vector system for targeting and transgene expression.
%J Journal of general virology
%V 98
%N 9
%@ 1465-2099
%C Reading
%I Soc.
%M DKFZ-2017-04637
%P 2248 - 2257
%D 2017
%X Viruses from the diverse family of Paramyxoviridae include important pathogens and are applied in gene therapy and for cancer treatment. The Tupaia paramyxovirus (TPMV), isolated from the kidney of a tree shrew, does not infect human cells and neutralizing antibodies against other Paramyxoviridae do not cross-react with TPMV. Here, we present a vector system for de novo generation of infectious TPMV that allows for insertion of additional genes as well as targeting using antibody single-chain variable fragments. We show that the recombinant TPMV specifically infect cells expressing the targeted receptor and replicate in human cells. This vector system provides a valuable tool for both basic research and therapeutic applications.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:28809150
%R 10.1099/jgv.0.000887
%U https://inrepo02.dkfz.de/record/128621