TY  - JOUR
AU  - Engeland, Christine
AU  - Bossow, Sascha
AU  - Hudacek, Andrew W
AU  - Hoyler, Birgit
AU  - Förster, Judith
AU  - Veinalde, Rūta
AU  - Jäger, Dirk
AU  - Cattaneo, Roberto
AU  - Ungerechts, Guy
AU  - Springfeld, Christoph
TI  - A Tupaia paramyxovirus vector system for targeting and transgene expression.
JO  - Journal of general virology
VL  - 98
IS  - 9
SN  - 1465-2099
CY  - Reading
PB  - Soc.
M1  - DKFZ-2017-04637
SP  - 2248 - 2257
PY  - 2017
AB  - Viruses from the diverse family of Paramyxoviridae include important pathogens and are applied in gene therapy and for cancer treatment. The Tupaia paramyxovirus (TPMV), isolated from the kidney of a tree shrew, does not infect human cells and neutralizing antibodies against other Paramyxoviridae do not cross-react with TPMV. Here, we present a vector system for de novo generation of infectious TPMV that allows for insertion of additional genes as well as targeting using antibody single-chain variable fragments. We show that the recombinant TPMV specifically infect cells expressing the targeted receptor and replicate in human cells. This vector system provides a valuable tool for both basic research and therapeutic applications.
LB  - PUB:(DE-HGF)16
C6  - pmid:28809150
DO  - DOI:10.1099/jgv.0.000887
UR  - https://inrepo02.dkfz.de/record/128621
ER  -