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@ARTICLE{Safian:130461,
      author       = {M. F. Safian$^*$ and N. Zinn$^*$ and J. Seidler$^*$ and W.
                      D. Lehmann$^*$},
      title        = {{M}icroquantification of phospholipid classes by stable
                      isotope dilution and nano{ESI} mass spectrometry.},
      journal      = {Fresenius' journal of analytical chemistry},
      volume       = {408},
      number       = {27},
      issn         = {1618-2650},
      address      = {Berlin},
      publisher    = {Springer56756},
      reportid     = {DKFZ-2017-05540},
      pages        = {7663 - 7667},
      year         = {2016},
      abstract     = {A new method for microquantification of phospholipid
                      classes by nanoelectrospray mass spectrometry and stable
                      isotope dilution is presented. The method covers the sum of
                      phosphatidylcholine and sphingomyelin and in addition
                      selectively quantifies phosphatidylethanolamine,
                      phosphatidylserine, and phosphatidylinositol. A phospholipid
                      class is quantified together with its corresponding
                      lyso-species due to the presence of a common head group.
                      Phospholipids are extracted from tissue lysates, hydrolysed
                      by hydrofluoric acid, and the liberated polar head groups
                      choline, ethanolamine, serine, and inositol are quantified
                      by nanoelectrospray mass spectrometry using
                      deuterium-labeled analogs of the head groups as internal
                      standards. The method is applied to tissue samples of a
                      gastrointestinal tumor and of corresponding non-affected
                      control tissue. In the tumor sample, the abovementioned
                      phospholipids were found at roughly threefold elevated
                      concentrations with a virtually unaltered relative abundance
                      profile.},
      cin          = {W160},
      ddc          = {540},
      cid          = {I:(DE-He78)W160-20160331},
      pnm          = {312 - Functional and structural genomics (POF3-312)},
      pid          = {G:(DE-HGF)POF3-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:27515797},
      doi          = {10.1007/s00216-016-9859-3},
      url          = {https://inrepo02.dkfz.de/record/130461},
}