Journal Article

DKFZ-2017-05540

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Microquantification of phospholipid classes by stable isotope dilution and nanoESI mass spectrometry.

Safian, M. F. (First author)DKFZ* ; Zinn, N.DKFZ* ; Seidler, J.DKFZ* ; Lehmann, W. D. (Last author)DKFZ*

2016
Springer56756 Berlin

This record in other databases:  

Please use a persistent id in citations: doi:10.1007/s00216-016-9859-3

Abstract: A new method for microquantification of phospholipid classes by nanoelectrospray mass spectrometry and stable isotope dilution is presented. The method covers the sum of phosphatidylcholine and sphingomyelin and in addition selectively quantifies phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol. A phospholipid class is quantified together with its corresponding lyso-species due to the presence of a common head group. Phospholipids are extracted from tissue lysates, hydrolysed by hydrofluoric acid, and the liberated polar head groups choline, ethanolamine, serine, and inositol are quantified by nanoelectrospray mass spectrometry using deuterium-labeled analogs of the head groups as internal standards. The method is applied to tissue samples of a gastrointestinal tumor and of corresponding non-affected control tissue. In the tumor sample, the abovementioned phospholipids were found at roughly threefold elevated concentrations with a virtually unaltered relative abundance profile.

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Contributing Institute(s):

1. Molekulare Strukturanalyse (W160)

Research Program(s):

1. 312 - Functional and structural genomics (POF3-312) (POF3-312)

Appears in the scientific report 2016

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Database coverage:
; BIOSIS Previews ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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