%0 Journal Article
%A Weber, Claudia E M
%A Luo, Chonglin
%A Hotz-Wagenblatt, Agnes
%A Gardyan, Adriane
%A Kordaß, Theresa
%A Holland-Letz, Tim
%A Osen, Wolfram
%A Eichmüller, Stefan
%T miR-339-3p Is a Tumor Suppressor in Melanoma.
%J Cancer research
%V 76
%N 12
%@ 1538-7445
%C Philadelphia, Pa.
%I AACR
%M DKFZ-2017-05906
%P 3562 - 3571
%D 2016
%X Determinants of invasion and metastasis in cancer remain of great interest to define. Here, we report the definition of miR-339-3p as a novel tumor suppressive microRNA that blocks melanoma cell invasion without affecting cell survival. miR-339-3p was identified by a comprehensive functional screen of a human miRNA mimetic library in a cell-based assay for invasion by the melanoma cell line A375. miR-339-3p was determined as a strong inhibitor of invasion differentially expressed in melanoma cells and healthy melanocytes. MCL1 was defined as a target for downregulation by miR-339-3p, functioning through direct interaction with the 3' untranslated region of MCL1 mRNA. Blocking miR-339-3p by an antagomiR was sufficient to increase melanoma cell invasion, an effect that could be phenocopied by RNAi-mediated silencing of MCL1. In vivo studies established that miR-339-3p overexpression was sufficient to decrease lung colonization by A375 melanoma cells in NSG mice, relative to control cells. Overall, our results defined miR-339-3p as a melanoma tumor suppressor, the levels of which contributes to invasive aggressiveness. Cancer Res; 76(12); 3562-71. ©2016 AACR.
%K MIRN-339 microRNA, mouse (NLM Chemicals)
%K Mcl1 protein, mouse (NLM Chemicals)
%K MicroRNAs (NLM Chemicals)
%K Myeloid Cell Leukemia Sequence 1 Protein (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:27197185
%R 10.1158/0008-5472.CAN-15-2932
%U https://inrepo02.dkfz.de/record/130828