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| Home > Publications database > miR-339-3p Is a Tumor Suppressor in Melanoma. |
| Home > Publications database > miR-339-3p Is a Tumor Suppressor in Melanoma. |
| Journal Article | DKFZ-2017-05906 |
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2016
AACR
Philadelphia, Pa.
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Please use a persistent id in citations: doi:10.1158/0008-5472.CAN-15-2932
Abstract: Determinants of invasion and metastasis in cancer remain of great interest to define. Here, we report the definition of miR-339-3p as a novel tumor suppressive microRNA that blocks melanoma cell invasion without affecting cell survival. miR-339-3p was identified by a comprehensive functional screen of a human miRNA mimetic library in a cell-based assay for invasion by the melanoma cell line A375. miR-339-3p was determined as a strong inhibitor of invasion differentially expressed in melanoma cells and healthy melanocytes. MCL1 was defined as a target for downregulation by miR-339-3p, functioning through direct interaction with the 3' untranslated region of MCL1 mRNA. Blocking miR-339-3p by an antagomiR was sufficient to increase melanoma cell invasion, an effect that could be phenocopied by RNAi-mediated silencing of MCL1. In vivo studies established that miR-339-3p overexpression was sufficient to decrease lung colonization by A375 melanoma cells in NSG mice, relative to control cells. Overall, our results defined miR-339-3p as a melanoma tumor suppressor, the levels of which contributes to invasive aggressiveness. Cancer Res; 76(12); 3562-71. ©2016 AACR.
Keyword(s): MIRN-339 microRNA, mouse ; Mcl1 protein, mouse ; MicroRNAs ; Myeloid Cell Leukemia Sequence 1 Protein
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