TY  - JOUR
AU  - Weber, Claudia E M
AU  - Luo, Chonglin
AU  - Hotz-Wagenblatt, Agnes
AU  - Gardyan, Adriane
AU  - Kordaß, Theresa
AU  - Holland-Letz, Tim
AU  - Osen, Wolfram
AU  - Eichmüller, Stefan
TI  - miR-339-3p Is a Tumor Suppressor in Melanoma.
JO  - Cancer research
VL  - 76
IS  - 12
SN  - 1538-7445
CY  - Philadelphia, Pa.
PB  - AACR
M1  - DKFZ-2017-05906
SP  - 3562 - 3571
PY  - 2016
AB  - Determinants of invasion and metastasis in cancer remain of great interest to define. Here, we report the definition of miR-339-3p as a novel tumor suppressive microRNA that blocks melanoma cell invasion without affecting cell survival. miR-339-3p was identified by a comprehensive functional screen of a human miRNA mimetic library in a cell-based assay for invasion by the melanoma cell line A375. miR-339-3p was determined as a strong inhibitor of invasion differentially expressed in melanoma cells and healthy melanocytes. MCL1 was defined as a target for downregulation by miR-339-3p, functioning through direct interaction with the 3' untranslated region of MCL1 mRNA. Blocking miR-339-3p by an antagomiR was sufficient to increase melanoma cell invasion, an effect that could be phenocopied by RNAi-mediated silencing of MCL1. In vivo studies established that miR-339-3p overexpression was sufficient to decrease lung colonization by A375 melanoma cells in NSG mice, relative to control cells. Overall, our results defined miR-339-3p as a melanoma tumor suppressor, the levels of which contributes to invasive aggressiveness. Cancer Res; 76(12); 3562-71. ©2016 AACR.
KW  - MIRN-339 microRNA, mouse (NLM Chemicals)
KW  - Mcl1 protein, mouse (NLM Chemicals)
KW  - MicroRNAs (NLM Chemicals)
KW  - Myeloid Cell Leukemia Sequence 1 Protein (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:27197185
DO  - DOI:10.1158/0008-5472.CAN-15-2932
UR  - https://inrepo02.dkfz.de/record/130828
ER  -