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@ARTICLE{Weigel:130847,
author = {C. Weigel$^*$ and M. R. Veldwijk and C. C. Oakes$^*$ and P.
Seibold$^*$ and A. Slynko$^*$ and D. B. Liesenfeld$^*$ and
M. Rabionet$^*$ and S. Hanke$^*$ and F. Wenz and E. Sperk
and A. Benner$^*$ and C. Rösli$^*$ and R. Sandhoff$^*$ and
Y. Assenov$^*$ and C. Plass$^*$ and C. Herskind and J.
Chang-Claude$^*$ and P. Schmezer$^*$ and O. Popanda$^*$},
title = {{E}pigenetic regulation of diacylglycerol kinase alpha
promotes radiation-induced fibrosis.},
journal = {Nature Communications},
volume = {7},
number = {NN},
issn = {2041-1723},
address = {London},
publisher = {Nature Publishing Group},
reportid = {DKFZ-2017-05925},
pages = {10893 -},
year = {2016},
abstract = {Radiotherapy is a fundamental part of cancer treatment but
its use is limited by the onset of late adverse effects in
the normal tissue, especially radiation-induced fibrosis.
Since the molecular causes for fibrosis are largely unknown,
we analyse if epigenetic regulation might explain
inter-individual differences in fibrosis risk. DNA
methylation profiling of dermal fibroblasts obtained from
breast cancer patients prior to irradiation identifies
differences associated with fibrosis. One region is
characterized as a differentially methylated enhancer of
diacylglycerol kinase alpha (DGKA). Decreased DNA
methylation at this enhancer enables recruitment of the
profibrotic transcription factor early growth response 1
(EGR1) and facilitates radiation-induced DGKA transcription
in cells from patients later developing fibrosis.
Conversely, inhibition of DGKA has pronounced effects on
diacylglycerol-mediated lipid homeostasis and reduces
profibrotic fibroblast activation. Collectively, DGKA is an
epigenetically deregulated kinase involved in radiation
response and may serve as a marker and therapeutic target
for personalized radiotherapy.},
keywords = {EGR1 protein, human (NLM Chemicals) / Early Growth Response
Protein 1 (NLM Chemicals) / RNA, Messenger (NLM Chemicals) /
Diacylglycerol Kinase (NLM Chemicals)},
cin = {C010 / C020 / C060 / G110 / G131 / A010 / V960},
ddc = {500},
cid = {I:(DE-He78)C010-20160331 / I:(DE-He78)C020-20160331 /
I:(DE-He78)C060-20160331 / I:(DE-He78)G110-20160331 /
I:(DE-He78)G131-20160331 / I:(DE-He78)A010-20160331 /
I:(DE-He78)V960-20160331},
pnm = {313 - Cancer risk factors and prevention (POF3-313)},
pid = {G:(DE-HGF)POF3-313},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:26964756},
pmc = {pmc:PMC4792958},
doi = {10.1038/ncomms10893},
url = {https://inrepo02.dkfz.de/record/130847},
}