%0 Journal Article
%A Lv, Na
%A Hao, Shuai
%A Luo, Chonglin
%A Abukiwan, Alia
%A Hao, Ying
%A Gai, Fei
%A Huang, Weiwei
%A Huang, Lingyun
%A Xiao, Xueyuan
%A Eichmüller, Stefan
%A He, Dacheng
%T miR-137 inhibits melanoma cell proliferation through downregulation of GLO1.
%J Science in China / C
%V 61
%N 5
%@ 1869-1889
%C Heidelberg
%I Springer37831
%M DKFZ-2018-00237
%P 541-549
%D 2018
%Z 2018 May;61(5):541-549
%X Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma cells. Bioinformatics analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter assay. Quantitative RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 expression. Furthermore, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 overexpression. Re-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:29307109
%R 10.1007/s11427-017-9138-9
%U https://inrepo02.dkfz.de/record/132559