Journal Article

DKFZ-2018-00237

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png miR-137 inhibits melanoma cell proliferation through downregulation of GLO1.

Lv, N. ; Hao, S. ; Luo, C. ; Abukiwan, A.DKFZ* ; Hao, Y. ; Gai, F. ; Huang, W. ; Huang, L. ; Xiao, X. ; Eichmüller, S.DKFZ* ; He, D.

2018
Springer37831 Heidelberg

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Please use a persistent id in citations: doi:10.1007/s11427-017-9138-9

Abstract: Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma cells. Bioinformatics analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter assay. Quantitative RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 expression. Furthermore, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 overexpression. Re-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.

Note: 2018 May;61(5):541-549

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Contributing Institute(s):

1. GMP Einheit Zelluläre Therapie (G182)

Research Program(s):

1. 317 - Translational cancer research (POF3-317) (POF3-317)

Appears in the scientific report 2018

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Database coverage:
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