TY  - JOUR
AU  - Lv, Na
AU  - Hao, Shuai
AU  - Luo, Chonglin
AU  - Abukiwan, Alia
AU  - Hao, Ying
AU  - Gai, Fei
AU  - Huang, Weiwei
AU  - Huang, Lingyun
AU  - Xiao, Xueyuan
AU  - Eichmüller, Stefan
AU  - He, Dacheng
TI  - miR-137 inhibits melanoma cell proliferation through downregulation of GLO1.
JO  - Science in China / C
VL  - 61
IS  - 5
SN  - 1869-1889
CY  - Heidelberg
PB  - Springer37831
M1  - DKFZ-2018-00237
SP  - 541-549
PY  - 2018
N1  - 2018 May;61(5):541-549
AB  - Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma cells. Bioinformatics analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter assay. Quantitative RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 expression. Furthermore, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 overexpression. Re-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.
LB  - PUB:(DE-HGF)16
C6  - pmid:29307109
DO  - DOI:10.1007/s11427-017-9138-9
UR  - https://inrepo02.dkfz.de/record/132559
ER  -