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@ARTICLE{Lv:132559,
author = {N. Lv and S. Hao and C. Luo and A. Abukiwan$^*$ and Y. Hao
and F. Gai and W. Huang and L. Huang and X. Xiao and S.
Eichmüller$^*$ and D. He},
title = {mi{R}-137 inhibits melanoma cell proliferation through
downregulation of {GLO}1.},
journal = {Science in China / C},
volume = {61},
number = {5},
issn = {1869-1889},
address = {Heidelberg},
publisher = {Springer37831},
reportid = {DKFZ-2018-00237},
pages = {541-549},
year = {2018},
note = {2018 May;61(5):541-549},
abstract = {Late-stage melanoma is refractory to current therapies.
MicroRNAs (miRNAs) can modulate many physiological and
pathological processes of melanoma. Studies have
demonstrated that miR-137 acts as a tumor suppressor by
inhibiting the proliferation of melanoma cells through
targeting multiple mRNAs. The glyoxalase system member
glyoxalase 1 (GLO1) is the principal scavenging enzyme of
methylglyoxal (MG), a toxic byproduct of glycolysis.
Using35S in vivo/vitro labelling analysis for dynamic
proteomics (SiLAD), we found that miR-137 downregulated the
expression of GLO1 in melanoma cells. Bioinformatics
analysis predicted that GLO1 is a direct target of miR-137.
This was validated by dual luciferase reporter assay.
Quantitative RT-PCR (qRT-PCR) and western blot analysis
indicated that miR-137 could decrease endogenous GLO1
expression. Furthermore, siRNA targeting of GLO1 mimicked
inhibition of melanoma cell proliferation caused by miR-137
overexpression. Re-expression of GLO1 was able to restore
miR-137-mediated suppression of melanoma cell proliferation.
Therefore, these results suggest that miR-137 inhibits the
proliferation of melanoma cells by targeting GLO1.},
cin = {G182},
ddc = {570},
cid = {I:(DE-He78)G182-20160331},
pnm = {317 - Translational cancer research (POF3-317)},
pid = {G:(DE-HGF)POF3-317},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29307109},
doi = {10.1007/s11427-017-9138-9},
url = {https://inrepo02.dkfz.de/record/132559},
}