Journal Article

DKFZ-2018-01137

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png DNA methylation-based reclassification of olfactory neuroblastoma.

Capper, D. (First author)DKFZ* ; Engel, N. W. ; Stichel, D.DKFZ* ; Lechner, M. ; Glöss, S.DKFZ* ; Schmid, S.DKFZ* ; Koelsche, C.DKFZ* ; Schrimpf, D.DKFZ* ; Niesen, J. ; Wefers, A. K.DKFZ* ; Jones, D.DKFZ* ; Sill, M.DKFZ* ; Weigert, O. ; Ligon, K. L. ; Olar, A. ; Koch, A. ; Forster, M. ; Moran, S. ; Tirado, O. M. ; Sáinz-Jaspeado, M. ; Mora, J. ; Esteller, M. ; Alonso, J. ; Del Muro, X. G. ; Paulus, W. ; Felsberg, J.DKFZ* ; Reifenberger, G.DKFZ* ; Glatzel, M. ; Frank, S. ; Monoranu, C. M. ; Lund, V. J. ; von Deimling, A.DKFZ* ; Pfister, S.DKFZ* ; Buslei, R. ; Ribbat-Idel, J. ; Perner, S. ; Gudziol, V. ; Meinhardt, M. ; Schüller, U.

2018
Springer Berlin

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Please use a persistent id in citations: doi:10.1007/s00401-018-1854-7

Abstract: Olfactory neuroblastoma/esthesioneuroblastoma (ONB) is an uncommon neuroectodermal neoplasm thought to arise from the olfactory epithelium. Little is known about its molecular pathogenesis. For this study, a retrospective cohort of n = 66 tumor samples with the institutional diagnosis of ONB was analyzed by immunohistochemistry, genome-wide DNA methylation profiling, copy number analysis, and in a subset, next-generation panel sequencing of 560 tumor-associated genes. DNA methylation profiles were compared to those of relevant differential diagnoses of ONB. Unsupervised hierarchical clustering analysis of DNA methylation data revealed four subgroups among institutionally diagnosed ONB. The largest group (n = 42, 64%, Core ONB) presented with classical ONB histology and no overlap with other classes upon methylation profiling-based t-distributed stochastic neighbor embedding (t-SNE) analysis. A second DNA methylation group (n = 7, 11%) with CpG island methylator phenotype (CIMP) consisted of cases with strong expression of cytokeratin, no or scarce chromogranin A expression and IDH2 hotspot mutation in all cases. T-SNE analysis clustered these cases together with sinonasal carcinoma with IDH2 mutation. Four cases (6%) formed a small group characterized by an overall high level of DNA methylation, but without CIMP. The fourth group consisted of 13 cases that had heterogeneous DNA methylation profiles and strong cytokeratin expression in most cases. In t-SNE analysis, these cases mostly grouped among sinonasal adenocarcinoma, squamous cell carcinoma, and undifferentiated carcinoma. Copy number analysis indicated highly recurrent chromosomal changes among Core ONB with a high frequency of combined loss of chromosome 1-4, 8-10, and 12. NGS sequencing did not reveal highly recurrent mutations in ONB, with the only recurrently mutated genes being TP53 and DNMT3A. In conclusion, we demonstrate that institutionally diagnosed ONB are a heterogeneous group of tumors. Expression of cytokeratin, chromogranin A, the mutational status of IDH2 as well as DNA methylation patterns may greatly aid in the precise classification of ONB.

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Contributing Institute(s):

1. KKE Neuropathologie (G380)
2. DKTK Heidelberg (L101)
3. B062 Pädiatrische Neuroonkologie (B062)
4. DKTK Berlin (L201)
5. DKTK Essen (L401)

Research Program(s):

1. 317 - Translational cancer research (POF3-317) (POF3-317)

Appears in the scientific report 2018

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Database coverage:
; BIOSIS Previews ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 10 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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